Study on the Expression and Immunogenicity of Chimeric Rabbit Hemorrhagic Disease Virus-like Particles as Carriers for B-cell Epitope of Foot-and-mouth Disease Virus

doi:10.7668/hbnxb.2015.01.023

Abstract

Abstract: To explore the capacity of antigen presentation of the foreign B-cell epitopes by virus-like particles (VLPs) of rabbit hemorrhagic (RHDV), the VLPs of RHDV displaying B-cell epitope of foot-and-mouth disease virus (FMDV) were constructed.The sequences of a FMDV B-cell epitope were fused to the N-terminal, C-terminal and inserted between 306th and 307th aa of capsid protein of RHDV.The fused genes were cloned into the donor vector pFastBac^TMHTA and three recombinant baculoviruses (rAc-VP60-2F, rAc-VP60-306F, rAc-VP60-578F) were constructed using Bac-to-Bac baculovirus expression system.These recombinant proteins were expressed effectively in insect cells as confirmed by IFA, SDS-PAGE and Western Blot.The immunogenicity of all chimeric VLPs was examined in mice.The results indicated the recombinant proteins could react with both anti-VP60 monoclonal antibody and anti-FMDV polyclonal antibody.And all recombinant proteins could self-assemble into VLPs by electron microscopy analysis.Furthermore, all chimeric VLPs were able to induce strong VP60-specific antibodies responses.In addition, there were significant differences of peptide-specific IgG antibodies induced by chimeric proteins, compared to that of VP60 group.The significant levels of serum antibodies against B-cell epitopes of FMDV demonstrated the feasibility of RHDV-VLP serving as a presentation carrier for foreign B-cell epitopes.

Key words: RHDV, Virus-like particles, FMDV, B-cell epitope, Vector

CLC Number:

SHENG Rong, SONG Yan-hua, HU Bo, FAN Zhi-yu, JIANG Ping, XUE Jia-bin, WEI Hou-jun, WANG Fang. Study on the Expression and Immunogenicity of Chimeric Rabbit Hemorrhagic Disease Virus-like Particles as Carriers for B-cell Epitope of Foot-and-mouth Disease Virus[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(1): 142-149. doi: 10.7668/hbnxb.2015.01.023.

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