Abstract:
Bacterial canker is the quarantine tomato seed-borne disease, it is rapid to detect the pathogen from the seed so that the disease can be controlled timely and effectivly and reduced the disease losses.The total genome DNA of Clavibacter michiganensis subsp. Michiganensis was extracted by Bacterial Genomic DNA Extraction Kit, then used for PCR with universal primer.A pair of primers ClaF1-ClaF2 was finally chose by screening specific PCR primers as rapid detection of Clavibacter michiganensis subsp. Michiganensis.The PCR assay was proved to be sensitive for detection the bacteria in the water of soaking seed which was carried bacterial artificially sufficiently reached to 1×105 CFU/mL.It was a specificity and high sensitivity detection method.With the PCR rapid detection method, the result showed that Heibei tomato, Fenhong tomato and Baoluota of 22 tomato varieties carried with Clavibacter michiganensis subsp. Michiganensis, others were not detected.This method was used for directly detecting the seeds, without isolating pathogens, fast and conveniently.
Key words:
Bacterial canker of tomato,
Rapid detection of PCR,
Seed-borne
CLC Number:
TIAN Xiao-yan, ZHANG Qing-ping, WANG Yan-chun, XI Xian-mei, BAI Hai. Rapid Detection of Clavibacter michiganensis subsp. Michiganensis in Inner Mongolia Area Using PCR and Testing of Seed Surface Borne Bacteria of 22 Tomato Varieties[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(1): 150-153. doi: 10.7668/hbnxb.2015.01.024.