Abstract: The purpose of this study was to determine whether Pseudorabies virus (PRV) infection caused by wild strain occurred at the farms where PRV gene deletion vaccine was inoculated under normal immune procedure in some areas of Henan Province and whether variation had occurred in gE gene which was one of the most important virulence genes of PRV.Samples from swine with suspicious PRV infection were collected from Nanyang, Zhoukou and other regions of Henan and detected by PCR.PRV-positive samples were inoculated into swine testis cells and sub-cultured six times.The complete genomes of gE of 9 PRV strains were cloned, sequenced and analyzed.The result showed that the length of gE genomes of the 9 strains were 1 862 bp.The homologies between the 9 strains were 99.9% -100% and the homologies with other strains were 97.5% -99.6%.Phylogenetic tree showed the 9 PRV strains belonged to a relatively independent sub-branch, and contained 2 amino acid insertions.The replacement of amino acid occurred at the locus of 448 and the locus of 510.It indicated that all of the isolated PRV strains were wild isolates, which were related with the new prevalence.The variation had occurred in gE gene of these isolates.

Key words: Pseudorabies virus, gE gene, Cloning, Sequence analysis

CLC Number: 

• S828