JA Mediated Signaling Pathway Regulate Plant Defense Responses of Root Knot Nematode

doi:10.7668/hbnxb.2014.03.042

Abstract

Abstract: Nematode parasites achieve chronic infection by overcoming the innate immunity system of the hosts.In plants, the role of JA to innate immunity is well established in the leaves.The role of JA in defending against parasitic root knot nematode is largely unknown.Here, we showed the root knot nematode Meloidogyne incognita infection induced Allene Oxide Synthase1 (AOS1) and OPR3 (12- oxo-Phytodienoic Acid Reductase 3) dependent JA and Jasmonate Resistant1 (JAR1) dependent JA-Ile biosynthesis.The nematode reproduction ability was significantly lower on the JA-Ile deficient roots(aos1, opr3, jar1) than those of wild types.Two JA signal transduction mutants, the coronatine insensitive1 (coi1) and the JA insensitive1 (jin1), showed wile type susceptibility to the nematode infection.The decreasing nematode reproduction ability on the roots of jar1, specifically missing JA-Ile, could be rescued by exogenous application of JA-Ile.JA synthesis pathway is important in prouss of the nematode infect Arabidopsis root.

Key words: Arabidopsis thaliana, Jasmonic acid, Signaling pathway, Root knot nematode, Mutant, Innate immunity

CLC Number:

Q945

WANG Jing, WU Xiao-hong, Qilemuge, Terigele, QI Zhi, KANG Yan. JA Mediated Signaling Pathway Regulate Plant Defense Responses of Root Knot Nematode[J]. journal1, 2014, 29(3): 234-237. doi: 10.7668/hbnxb.2014.03.042.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2014.03.042

http://www.hbnxb.net/EN/Y2014/V29/I3/234

References

[1] Noir S,Bmer M,Takahashi N,et al.Jasmonate controls leaf growth by repressing cell proliferation and the onset of endoreduplication while maintaining a potential stand-by mode [J].Plant Physiology,2013,161(4):1930-1951.
[2] Wasternack C.Jasmonates:An update on biosynthesis,signal transduction and action in plant stress response,growth and development [J].Annals of Botany,2007,100(4):681-697.
[3] Yan Y,Stolz S,Chételat A,et al.A downstream mediator in the growth repression limb of the jasmonate pathway [J].The Plant Cell,2007,19(8):2470-2483.
[4] Ziegler J,Stenzel I,Hause B,et al.Molecular cloning of allene oxide cyclase-The enzyme establishing the stereochemistry of octadecanoids and jasmonates[J].The Journal of Biological Chemistry,2000,275(25):19132-19138.
[5] Schaller F,Biesgen C,Müssig C, et al.12-oxophytodienoate reductase 3(OPR3) is the isoenzyme involved in jasmonate biosynthesis[J].Planta,2000,210(6):979-984.
[6] Koo A J,Chung H S,Kobayashi Y,et al.Identification of a peroxisomal acyl-activating enzyme involved in the biosynthesis of jasmonic acid in Arabidopsis[J].The Journal of Biological Chemistry,2006,281(44):33511-33520.
[7] Schilmiller A L,Koo A J,Howe G A.Functional diversification of acyl-coenzyme A oxidases in jasmonic acid biosynthesis and action [J].Plant Physiology,2007,143(2):812-824.
[8] Staswick P E,Tiryaki I.The oxylipin signal jasmonic acid is activated by an enzyme that conjugates it to isoleucine in Arabidopsis[J].The Plant Cell,2004,16(8):2117-2127.
[9] McConn M,Creelman R A,Bell E, et al.Jasmonate is essential for insect defense in Arabidopsis[J].Proceedings of the National Academy of Sciences of the United States of America,1997,94(10):5473-5477.
[10] Jasmer D P,Goverse A,Smart G.Parasitic nematode interactions with mammals and plants [J].Annual Review of Phytopathology,2003,41:245-270.
[11] Sasser J N,Freckman D W.A world perspective on nematology-the role of the society [J].Journal of Nematology,1986,18(4):596.
[12] Kyndt T,Vieira P,Gheysen G,et al.Nematode feeding sites:unique organs in plant roots [J].Planta,2013,238(5):807-818.
[13] Davis E L,Hussey R S,Baum T J.Getting to the roots of parasitism by nematodes [J].Trends in Parasitology,2004,20(3):134-141.
[14] von Malek B,van der Graaff E,Schneitz K,et al.The Arabidopsis male-sterile mutant dde2-2 is defective in the ALLENE OXIDE SYNTHASE gene encoding one of the key enzymes of the jasmonic acid biosynthesis pathway [J].Planta,2002,216(1):187-192.
[15] Stintzi A,Browse J.The Arabidopsis male-sterile mutant,opr3,lacks the 12-oxophytodienoic acid reductase required for jasmonate synthesis [J].Proceedings of the National Academy of Sciences of the United States of America,2000,97(19):10625-10630.
[16] Ellis C,Karafyllidis I,Wasternack C,et al.The Arabidopsis mutant cev1 links cell wall signaling to jasmonate and ethylene responses[J].The Plant Cell,2002,14(7):1557-1566.
[17] Lorenzo O,Chico J M,Sánchez-Serrano J J,et al.Jasmonate-insensitive1 encodes a MYC transcription factor essential to discriminate between different jasmonate-regulated defense responses in Arabidopsis[J].The plant Cell,2004,16(7):1938-1950.
[18] Hammes U Z,Schachtman D P,Berg R H,et al.Nematode-induced changes of transporter gene expression in Arabidopsis roots [J].Molecular Plant-Microbe Interactions,2005,18(12):1247-1257.
[19] Staswick P E,Tiryaki I,Rowe M L.Jasmonate response locus JAR1 and several related Arabidopsis genes encode enzymes of the firefly luciferase superfamily that show activity on jasmonic,salicylic,and indole-3-acetic acids in an assay for adenylation [J].The Plant Cell,2002,14(6):1405-1415.
[20] Ellis C,Turner J G.A conditionally fertile coi1 allele indicates cross-talk between plant hormone signalling pathways in Arabidopsis thaliana seeds and young seedlings [J].Planta,2002,215(4):549-556.
[21] Laudert D,Weiler E W.Allene oxide synthase:a major control point in Arabidopsis thaliana octadecanoid signalling [J].The Plant Journal,1998,15(5):675-684.
[22] Sarkar D,Pandey S K,Sharma S.Cytokinins antagonize the jasmonates action on the regulation of potato(Solanum tuberosum) tuber formation in vitro [J].Plant Cell Tissue and Organ Culture,2006,87(3):285-295.
[23] Curtis R H.Do phytohormones influence nematode invasion and feeding site establishment [J].Nematology,2007,9(2):155-160.
[24] Shah J.Lipases lipases and lipid-modifying enzymes in plant disease resistance [J].Annual Review of Phytopathology,2005,43:229-260.
[25] Vellosillo T,Martínez M,López M A,et al.Oxylipins produced by the 9-lipoxygenase pathway in Arabidopsis regulate lateral root development and defense responses through a specific signaling cascade [J].The Plant Cell,2007,19(3):831-846.
[26] Prior A,Jones J T,Blok V C,et al.A surface-associated retinol-and fatty acid-binding protein(Gp-FAR-1) from the potato cyst nematode Globodera pallida :lipid binding activities,structural analysis and expression pattern [J].The Biochemical Journal,2001,356Pt2:387-394.

Please choose a citation manager

Content to export