Cloning and Bioinformatic Analysis of PGIPGene from Prunus caoyuan

doi:10.3969/j.issn.1000-7091.2012.06.009

Abstract

Abstract: Disease resistance mechanism was studied by methods of cloning of the PGIPgene in Prunus caoyuan． A DNA fragment about 1 kb was amplified from the genomic DNA of Prunus caoyuanleaves by PCR with a pair of specific primers based on the conserved sequences of the PGIPgenes of genus Prunus． Sequence analysis showed that the fragment contains a full coding region of 1 193 bp( GenBank accession: GU068977). This sequence had a full open reading frame encoding the PGIP，and eontained two exons interrupted by one intron． The total exons were comprised by 990 bp of deoxynucleotide encoding 330 amino acid． A conserved leucine-rich fragmenthad existed in the derived protein sequence． Sequencing analysis showed that it was 95% to 99% identical with the sequences of Prunus PGIPgenes including P． salicina，P． armeniaca，P． persica，P． mahaleb，P． mume． Phylogenic tree showed that genetic relationship within the genus was closer and between the genera was farther． A PGIPgene of Prunus caoyuanwas cloned． As a result，a gene resource was provided for molecular breeding of plants．

Key words: Prunus caoyuan, PGIPgene, Gene cloning, Bioinformatic

CLC Number:

YU Wen-quan, LIU Hai-rong, YANG Xiao-hua, ZHAO Heng-tian. Cloning and Bioinformatic Analysis of PGIPGene from Prunus caoyuan[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(6): 38-42. doi: 10.3969/j.issn.1000-7091.2012.06.009.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.3969/j.issn.1000-7091.2012.06.009

http://www.hbnxb.net/EN/Y2012/V27/I6/38

References

[1] DI Matteo A，Federici L，Mattei B， et al． The crystal structure of polygalacturonase-inhibiting protein( PGIP),a leucine-rich repeat protein involved in plant defense [J]． Proceedings of the National Academy of Sciences of the United States of America， 2003， 100: 10124-10128．
[2] Simpson C G，MacRae E，Gardner R C． Cloning of a polygalacturonase inhibiting protein fron Kiwifruit( Actinidia deliciosa) [J]． Plant Physiol， 1995， 108: 1748．
[3] Favaron F，D'Ovido R，Porceddu E， et al． Purification and molecular characterization of a soybean polygalacturonaseinhibiting protein [J]． Planta， 1994， 195: 80-87．
[4] Yao C，Conway W S，Sams C E． Purification and characterization of a polygalacturonase-inhibiting protein from apple fruit [J]． Phytopathology， 1995， 85: 1373-1377．
[5] Stotz H U，Powell A L T，Damon S E，et al． Molecular characterization of a polygalacturonase inhibitor from Pyrus communis L． cv． Bartlett [J]． Plant Physiology， 1993， 102: 133-138．
[6] Johnston D J，Ramanathan V，Williamson B． A protein from immature raspberry fruits which inhibits endopolygalacturonases from Botrytis cinereaand other micro-organisms[J]． Journal of Experimental Botany， 1999， 44: 971-976．
[7] 李广平，房经贵，蔡斌华，等．梅PGIP 基因的克隆及序列分析[J]．园艺学报， 2006， 33( 1) : 125-127．
[8] 李广平，乔玉山，陶建敏，等．中国李PGIP 基因的克隆及序列分析[J]．西北植物学报，2006，26( 9) : 1870-1873．
[9] Jones D A， Jones I D G． The role of leucine-rich repeat proteins in plant defences [J]． Advances in Botanical Research， 1997， 24: 89-167．
[10] Brown A E． Relationship of endopolygalacturonase inhibitor activity to the rate of fungal rot development in apple fruits
[J]． Phytopathologische Zeitschrift， 1984， 111: 122-132．
[11] Gao S，Shain L． Activity of polygalacturonase produced by cryphonectria parasitica in chestnut bark and its inhibition by extracts from American and Chinese chestnut
[J]． Physiological and Molecular Plant Pathology， 1995， 46: 199-213．
[12] Cervone F，Hahn M G，De Lorenzo G． Host-pathogen interaction XXXIII A plant protein convoerts a fungal pathogenesis factor into an elicitor of plant defense responses
[J]． Plant Physio， 1989， 90: 542-548．
[13] Cervone F，De Lorenzo G，Darvll A， et al． Can Phaseolus PGIP inhibit pecti enzyms from microbes and plants?[J]． Phytochemisty， 1990， 292: 447-449．
[14] D'Ovidio R，Mattei B，Roberti S， et al． Polygalacturonases， polygalacturonase-inhibiting proteins and pectic oligomers in plant-pathogen interactions [J]． Biochimica et Biophysica Acta， 2004， 1696: 237-244．
[15] Powell A L T， van Kan J， ten Have A， et al． Transgenic expression of pear PGIP in tomato limits fungal colonization[J]． Molecular Plant-Microbe Interaction， 2000， 13: 942-950．
[16] Favaron F，Castiglioni C，D'Ovidio R，et al． Polygalacturonase inhibiting proteins from Allium porrum L． and their role in plant tissue against fungal endo-polygalacturonases[J]． Physiological and Molecular Plant Pathology， 1997， 50: 403-417．

Please choose a citation manager

Content to export