Isolation and Characterization of Ty1-copia Retrotransposon Sequence from Paeonia suffruticosaAndrews

doi:10.3969/j.issn.1000-7091.2012.06.007

Abstract

Abstract: With degenerate primer， the Ty1-copia-like retrotransposon sequence fragments were amplified from peony( Paeonia suffruticosaAndrews． ) genomic DNA and sequemced，getting a 240-bp PCR fragment which contains two open reading frames and encodes 63 amino acids． The results of BLAST in GenBank showed that the RT gene sequence of peony had more than 70% similarity with those of Lycium chinense and Beta vulgaris，etc． A phylogenetic analysis by MEGA and DNAstar software showed that the sequence had higher homology and closer paternity with those of deciduous woody trees such as Prunus mume，Populus ciliate，Camellia sinensisand Malus x domestica than those of herbaceous plants such as L． barbarum，Cicer arietinum，Lycopersicon esculentumand Fragaria viridis．

Key words: Paeonia suffruticosa, Retrotransposon, RTgene, Cloning

CLC Number:

S685.11

JIA Tian-tian, GUO Da-long, HOU Xiao-gai, LIU Gai-xiu, WANG Li-na, ZHANG Xiu-ming, LI Shuang-shuang. Isolation and Characterization of Ty1-copia Retrotransposon Sequence from Paeonia suffruticosaAndrews[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(6): 30-33. doi: 10.3969/j.issn.1000-7091.2012.06.007.

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