Abstract: Lilium brownii F.E.Brown ex Miellez,Brown lily,belongs to Lilium of Liliaeeae in planttaxonorny,is a endemic species and one of commonlly-used cross breeding parents of Lilium in China.L.brownii F.E.Brown ex Miellez var.virdulum Baker,Greenish lily,a variety of Lilium brownii,is also a well known medicinal and edible plant.Wild germplasm resources are the basic materials for genetic research and plant breeding.Howerever,due to Lilium brownii is scattered in the wild and its collection is generally only in flowering phase,it is a great difficulty for its scientific research.In the year before,researches on Lilium brownii especially molecular biology researches are still lacking in Guangdong province.In this study,total 199 Lilium brownii samples of 7 natural populations collected from representative mountain areas in Guangdong China were analyzed by RAPD markers,so as to provide good foundation for further natural germplasm resources and related studies of Lilium brownii populations,and provide good evidence of the discrimination of Brown lily and Greenish lily at molecular level.DNA from all samples was extracted from leaves and scales,which amplified clear,bright and steady RAPD bands.20 primers were screened from 273 random primers,and a total 433 DNA bands were amplified,430(99.3%) of which were polymorphic,the percentage of polymorphic bands(PPB) at the population level was 60.67% on average.The unweighted pair group method arithmetic average(UPGMA) clustery analysis of the RAPD data from 199 samples showed that all Brown lily samples and Greenish lily samples in the same population separated obviously at the value of 0.68 of genetic similarity coefficient,which was a good evidence of the discrimination of Brown lily and Greenish lily at molecular level.

Key words: Wheat immature embryos, Photo-thermo-sensitive male sterility, Carbon source, Concentration of herbicide, Genotype

CLC Number: 

• S512．03