Study on Optimization of Agrobacterium -mediated Transformation System of Cucumber

doi:10.7668/hbnxb.2015.05.019

Abstract

Abstract: For the purpose of improving the efficiency of Agrobacterium-mediated transformation of cucumber,the most suitable genotype was selected from Asian ecotype Changchunmici and European ecotype Poinset76 and Marketmore76.And then the Agrobacterium-mediated transformation system of cucumber was optimized with changing medium curing agent and adding antioxidant and organic additive into medium in different culture stage.The results indicated that the resistant buds differentiation of Changchunmici was the best of the three cucumber genotypes,Poinset76 and Marketmore76 were poorer.The resistant buds induction rates of three genotypes on medium gelled with gellan gum were increased by 28.21,19.71 and 15.39 percentage points respectively compared with those on medium gelled with agar.Adding 50 mg/L α-lipoic acid into co-culture medium could make the resistant buds induction rates of Changchunmici up to 66.67% and number of shoots per explants up to 1.32.Similarly,when adding 1.5 g/L casein hydrolysate into selection culture medium,the resistant buds induction rate and number of shoots per explants of Changchunmici were the highest,67.15% and 1.42 respectively.In conclusion,this article gained the optimization of transformation system that was using Changchunmici as appropriate genotype and gellan gam as medium curing agent,then adding 50 mg/L α-lipoic acid into co-culture medium or 1.5 g/L casein hydrolysate into selection culture medium based on previous Agrobacterium -mediated transformation system.This optimized transformation system very significantly improved the resistant buds induction rate and number of shoots per explants of cucumber and promoted the process of cucumber transformation.

Key words: Cucumber, Transformation, Genotype, Curing agent, Antioxidant, Organic additive

CLC Number:

S642.03

LI Lei, MENG Yong-jiao, ZHANG Lu, LOU Qun-feng, LI Ji, QIAN Chun-tao, CHEN Jin-feng. Study on Optimization of Agrobacterium -mediated Transformation System of Cucumber[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(5): 115-121. doi: 10.7668/hbnxb.2015.05.019.

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