Regeneration System Establishment from Cotyledons in Different Cucumber(Cucumis sativus L. ) Genotypes

doi:10.7668/hbnxb.20102.S1.013

Abstract

Abstract: The effect of genotype and phytohormone(6-BA,ABA) were studied by using six types of cucumber cotyledon as explants in this experiment. The results showed that the shoot regeneration frequency was different among the various cucumber genotypes. Jilinhangua has the highest shoot regeneration frequency which reached 97% and 5. 2 shoots per explant cultured in M7(MS + 1. 5 mg /L 6-BA + 0. 5 mg /L ABA + 2 mg /L AgNO₃ ) medium. The number of regenerate shoot was improved in the medium containing 6-BA,ABA and AgNO₃ compared to CK,and 6-BA was necessary and ABA was efficient to the shoot regeneration. Moreover the elongation of the regenerate shoot was significant in the medium containing 0. 1 mg /L 6-BA.

Key words: Cucumber, Cotyledon, Genotype, Regeneration

CLC Number:

S642.03

ZHANG Ruo-wei, GU Xing-fang, WANG Ye, ZHANG Sheng-ping, ZHANG Bao-xi. Regeneration System Establishment from Cotyledons in Different Cucumber(Cucumis sativus L. ) Genotypes[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(S1): 50-54. doi: 10.7668/hbnxb.20102.S1.013.

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References

[1] 佟新萍.黄瓜子叶下胚轴愈伤组织诱导及植株再生试验[J].石河子科技,1996,4:11-12.
[2] 吕德扬,Cocking E C.黄瓜子叶原生质体苗的再生[J].科学通报,1984,7:434-436.
[3] 张承妹,陆家安.黄瓜(Cucumis sativus L.)组织培养与诱导四倍体再生植株[J].上海农业学报,1995,11(3):31-36.
[4] 王艳蓉,陈丽梅,潘俊松,等.黄瓜子叶高效再生体系得建立与遗传转化[J].上海交通大学学报:农业科学版,2006,24(2):152-156.
[5] 赵军良,马蓉丽,李昌华.黄瓜子叶组织培养再生植株[J].山西农业科学,1996,24(1):39-41.
[6] 冯嘉玥,邹志荣,秦胜华,等.黄瓜子叶节高频再生体系建立及再生植株倍性观察[J].西北植物学报,2008,28(5):956-962.
[7] Burza.W,Malepszy S.Direct plant regeneration from leaf explants in cucumber(Cucumis sativus L.)is free of stable genetic variation[J].Plant breeding,1995,114(4):341-345.
[8] Punja Z K,Tang F A,Sarmento G G.Isolation,culture and plantlet regeneration from cotyledon and mesophyll protoplasts of two pickling cucumber(C.sativus L.)genotypes[J].Plnate Cell Reports,1990,9(2):61-64.
[9] 佐藤正孝*今西,茂*桶浦严.黄瓜胚轴及胚轴愈伤组织的植株分化[J].育种学杂志,1979,29(1):33-38.
[10] 赵泓,刘凡,姚磊.简单快捷建立高频黄瓜子叶离体再生体系[J].生物技术,2000,10(2):9-11.
[11] 梅茜,张兴国.黄瓜组织培养研究[J].西南农业大学学报,2002,24(3):266-267.
[12] Todd C W,Robert D L.In vitro adventitious shoot and root formation of cultivars and lines of Cucumis sativus L.[J].Hortscience,1981,16(6):759-760.
[13] 李建欣,李建吾,葛桂民.黄瓜子叶离体再生体系研究[J].长江蔬菜,2008,1:44-47.
[14] 贾金生,司龙亭,韩贵超,等.不同基因型黄瓜离体再生及其影响因素的研究[J].河南农业科学,2008,6:99-102.
[15] 范爱丽,孙艳,徐凌飞,等.黄瓜子叶节再生体系优化研究[J].西北农林科技大学学报:自然科学版,2006,34(9):69-73.
[16] 秦华伟,徐跃进,何丹,等.不同因素对黄瓜子叶再生植株影响的研究[J].湖北农业科学,2009,48(7):1548-1550.
[17] Paula P Chee.High frequency of somatic embryogenesis and recovery of fertile cucumber plants[J].HortScience,1990,25(7):792-793.
[18] Selvaraj N,Vasudevan A,Manickavasagam M,et al.In vitro organogenesis and plant formation in cucumber[J].Biologia Plantarum,2006,50(1):123-126.
[19] Selvaraj N,Vasudevan A,Manickavasagam M,et al.High frenquency shoot regeneration from cotyledon explants of cucumber via organogenesis[J].Scientia Horticulture,2007,112:2-8.
[20] Lou H,Kako S.Somatic embryogenesis and plant regeneration in cucumber[J].Hortscience,1994,29(8):906-909.
[21] Jin-Zhuo Dong,Shi-Rong Jia.High efficiency plant regeneration from cotyledons of watermelon(Citrullus vulgaris Schrad.)[J].Plant Cell Reports,1991,9:559-562.

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