The Choice of Photo-thermo-sensitive Male Sterile Wheat Receptor Through Partical Bombardment and the Optimization of Culture Conditions

doi:10.7668/hbnxb.2011.04.021

Abstract

Abstract: In order to choose the appropriate receptor of photo-thermo-sensitive male sterile wheat for bombardment,and optimize the Culture condition of immature embryos,We used immature embryos of 3 wheat lines,including photo-thermo-sensitive male sterile line BS210,BS366 and normal cultivar Jing 411,Carbon source,screening concentration and genotype were screened respectively.The study showed that the frequency of embryos callus formation is not significantly different between sucrose and maltose as the carbon source.While adding maltose in different mediums,the frequency of callus differentiation was higher than the medium adding sucrose.During screening callus that the bar gene had bombarded in,the optimal concentration of herbicides biolaphos was 2 mg/L.The seeding rate of three material was significantly different.The seeding rate of BS210 was higher than other plants,which is the best recipient for transformation.The research sets the foundation for the improvement of photo-thermo-sensitive male sterile wheat.

Key words: Maize, Inbred line, Water use efficiency, Physiological changes

CLC Number:

S513．01

JIA Ning, GAO Ju-lin, YU Xiao-fang, WANG Zhi-gang, SUN Ji-ying, HU Shu-ping, WANG Hai-yan, GAO Ying-bo, YE Jun. The Choice of Photo-thermo-sensitive Male Sterile Wheat Receptor Through Partical Bombardment and the Optimization of Culture Conditions[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(4): 121-125. doi: 10.7668/hbnxb.2011.04.021.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2011.04.021

http://www.hbnxb.net/EN/Y2011/V26/I4/121

References

[1] 贾大林. 关于对以节水为中心的大中型灌区改造规划的意见[A].西安:陕西科学技术出版社,2000.91-30.
[2] 杨涛,梁宗锁,薛吉全. 不同玉米品种水分利用效率的差异性研究[J].农业工程学报,2005,(10):21-25.doi:10.3321/j.issn:1002-6819.2005.10.006.
[3] 于晓芳,高聚林,宋国栋. 玉米叶片水分利用效率及其相关性状的研究[J].玉米科学,2008,(03):64-69.
[4] 山仑. 植物水分利用效率和半干旱地区农业用水[J].植物生理学通讯,1994,(01):61-66.
[5] 于晓芳,高聚林,宋国栋. 玉米叶片水分利用效率与叶绿素a荧光参数的关系[J].玉米科学,2009,(04):55-57,60.
[6] 冀天会,张灿军,杨子光. 冬小麦叶绿素荧光参数与品种抗旱性的关系[J].麦类作物学报,2005,(04):64-66.doi:10.3969/j.issn.1009-1041.2005.04.014.
[7] 李刚,王金艳. 玉米单叶水分利用效率的差异比较[J].杂粮作物,2006,(05):353-355.doi:10.3969/j.issn.2095-0896.2006.05.013.
[8] 杨青华,韩锦峰,刘华山. 液膜对棉花叶片蒸腾特性及水分利用效率的影响[J].华北农学报,2004,(04):96-99.doi:10.3321/j.issn:1000-7091.2004.04.026.
[9] 周志刚,陈明琦,藏健. 半干旱地区不同抗旱保水剂对玉米出苗和生长的影响[J].内蒙古农业科技,2007,(04):57-58.doi:10.3969/j.issn.1007-0907.2007.04.020.
[10] 张岁岐,山仑. 植物水分利用效率及其研究进展[J].干旱地区农业研究,2002,(04):1-5.doi:10.3321/j.issn:1000-7601.2002.04.001.
[11] 李国强,周吉,徐萍. 玉米抗旱节水产量性状相关及通径分析[J].华北农学报,2009,(增刊):131-136.
[12] 张振平,齐华,张悦. 水分胁迫对玉米光合速率和水分利用效率的影响[J].华北农学报,2009,(增刊):155-158.
[13] 刘化涛,黄学芳,黄明镜. 不同品种与种植密度对旱地玉米产量及水分利用效率的影响[J].山西农业科学,2010,(09):32-34.doi:10.3969/j.issn.1002-2481.2010.09.10.
[14] 樊修武,池宝亮,黄学芳. 不同水分梯度下玉米水分利用效率研究[J].山西农业科学,2008,(01):60-63.doi:10.3969/j.issn.1002-2481.2008.11.015.

[1]	SUN Ning, BIAN Shaofeng, YAN Weiping, XU Chen, CHEN Xifeng, SONG Hanglin, LI Fei, ZHANG Lihua, TAN Guobo, ZHAO Hongxiang. Synergistic Regulation Characteristics and Lodging Resistant Effect of Plant Growth Regulators on Canopy-Root of Spring Maize [J]. Acta Agriculturae Boreali-Sinica, 2025, 40(2): 56-66.
[2]	BAI Dongxing, CHEN Xuanyi, ZHANG Xiangqian, GE Guolong, WANG Manxiu, ZHANG Dejian, MENG Tiantian, LUO Fang, ZHANG Chuanling. Effects of Different Tillage Methods on Soil Moisture Dynamic Changes and Maize Yield [J]. Acta Agriculturae Boreali-Sinica, 2025, 40(2): 67-80.
[3]	CHEN Huafeng, ZHANG Jianing, ZHANG Xiao, YUAN Yue, LIU Xiufeng, LIU Dan. Evolution and Functional Analysis of Maize Gene ZmC₄NADP-ME [J]. Acta Agriculturae Boreali-Sinica, 2025, 40(2): 10-17.
[4]	ZHANG Xuting, ZHANG Hailong, ZHANG Jiaqian, LIU Yanan, ZHANG Dejian, FU Zengjuan, XUE Chunlei, ZHANG Sainan, SUN Fengcheng. Effects of Different Nitrogen Application Amounts of Foliar Fertilizers on Nitrogen Accumulation,Translocation and Utilization in Maize [J]. Acta Agriculturae Boreali-Sinica, 2025, 40(2): 117-126.
[5]	LAN Huiqing, MENG Tiantian, ZHANG Xiangqian, WANG Weini, ZHANG Jun, CHEN Liyu, LU Zhanyuan, SUN Xia. Effect of Nitrogen Application Levels on Soil Organic Nitrogen Fractions and Nitrogen Utilization Rates [J]. Acta Agriculturae Boreali-Sinica, 2025, 40(1): 166-176.
[6]	PENG Keyan, ZHAO Kainan, ZHOU Fabao, DIAO Yanbin, CHEN Guangzhou, LI Shengdong. Effects of Nitrogen Fertilizer Reduction and Postponing on Grain Yield and Nitrogen Absorption and Utilization of Wheat-Maize Double Cropping System [J]. Acta Agriculturae Boreali-Sinica, 2025, 40(1): 133-145.
[7]	KONG Ming, ZHU Jinjie, QI Xiantao, XIE Chuanxiao, LIU Changlin. Staining Method and Fluorescent Strain Construction of Fusarium verticillioides [J]. Acta Agriculturae Boreali-Sinica, 2024, 39(S1): 252-258.
[8]	HONG Ziqiang, ZHANG Zhengzhen, WANG Jia, ZHOU Tian, LI Fanguo, SU Ming, WU Hongliang, KANG Jianhong. Response Pattern of Spring Maize Yield,Photosynthetic Characteristics and Fluorescence Parameters to Phosphorus Dosage in Ningxia Irrigation Area [J]. Acta Agriculturae Boreali-Sinica, 2024, 39(6): 163-175.
[9]	QIAN Buxuan, PAN Hong, WANG Qi, CHEN Ziqi, YANG Yawen, XU Jieting, XIA Hanchao, ZHAO Rengui, LIU Xiangguo. *Identification and Efficiency Testing of Cas9 Variants Targeting NG PAM Sequences for Editing the ZmSCD* Gene in Maize** [J]. Acta Agriculturae Boreali-Sinica, 2024, 39(5): 9-14.
[10]	YANG Wanbang, WANG Xiaoyuan, YU Rong, DU Huiying, LIU Shengfeng, TIAN Mei, GUO Song, WEI Zhaohui. The Influence for Water and Nitrogenous Fertilizer Interaction on Quality, Yield and Absorption and Utilization of Nitrogen for Facility-cultivated Watermelon in Yellow River Irrigation Area of Ningxia [J]. Acta Agriculturae Boreali-Sinica, 2024, 39(4): 154-161.
[11]	XIA Ke, LUO Yanmu, HUANG Min, DU Hewei. Genome-wide Identification and Expression Pattern Analysis of DUF668 Family Members in Maize [J]. Acta Agriculturae Boreali-Sinica, 2024, 39(4): 20-30.
[12]	HUANG Huanhuan, AN Hongzhou, LI Kuiying, WANG Yanbing, GU Yi, QIAO Yake, GAO Zengyu. QTL Mapping of Silk Coloring Characteristics of Anthocyanins in Maize [J]. Acta Agriculturae Boreali-Sinica, 2024, 39(4): 31-36.
[13]	GUO Zhaoyang, YIN Yuhang, LIU Yu, XIE Yitong, PEI Yuhe, SONG Xiyun, ZHAO Meiai. *Cloning and Expression Pattern Analysis of ZmPAP26b* Gene in Maize under Drought Stress** [J]. Acta Agriculturae Boreali-Sinica, 2024, 39(4): 10-19.
[14]	JIANG Xiaomin, YANG Caihong, CUI Wenqiang, TIAN Kun. Effects of Different Cropping Patterns on Photosynthesis,Leaf Structure and Yield of Maize in Hexi Irrigation Area [J]. Acta Agriculturae Boreali-Sinica, 2024, 39(3): 124-134.
[15]	ZHANG Guiping, Mukti Marasini, LI Weiwei, ZHANG Fenglu. Response of Stalk Traits to Stalk Elasticity and Density Tolerance of Different Maize Cultivars [J]. Acta Agriculturae Boreali-Sinica, 2024, 39(2): 79-89.

Please choose a citation manager

Content to export