Cloning, Expression and Insecticidal Activity of cry7Ab8 Gene from Bacillus thuringiensis

doi:10.7668/hbnxb.2011.02.008

Abstract

Abstract: A cry7-type gene from Bt GWS7 strain was identified by full-length PCR method.The cry7Ab8 gene was amplified and cloned into pET28b.The recombinant plasmid pET cry7Ab8 Was transformed into E.coli BL21 (DE3),resulting BL21(pET cryTAb8).The SDS-PAGE results showed that the 130 kDa Cry7Ab8 protein Was expressed.An engineering strain BioHD7Ab8 was construced by transforming the shuttle vector pSXY422b containing cry7Ab8 into Bt acrystalliferous mutant HD73^-(cry^-).The bioassay results indicated that the Cry7Ab8 protein was highly toxic against Henosepilachnavigintioctomaculata larvae,and the LC₅₀ value was 548 ug/mL.

Key words: Bacillus thuringiensis, cryTAb8 gene, Gene cloning, Gene expression, Insecticidal activity

CLC Number:

Q786

FENG Jing-jing, GUO Wei, LIU Ting-hui, SUN Yong-xiang, SUN Wei-ming, XU Da-qing. Cloning, Expression and Insecticidal Activity of cry7Ab8 Gene from Bacillus thuringiensis[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(2): 35-38. doi: 10.7668/hbnxb.2011.02.008.

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