Cloning of ORFS Gene of PRRS Virus and Construction of Its Baculovirus Expression Vector

doi:10.7668/hbnxb.2009.01.019

Abstract

Abstract: According to the published genome sequences of PRRSV, one pair of spec cprimers were designed toamplify ORF5 gene of L211 strain of GAMS U province by RT-PCR. The ampl}ied fragment was cloned into pFastBacHTAdonor plasmid. The recombinant pFastBacHTA was identified lry digestion with clonuclease enzyme, PCR amplificationand sequencing.Then the purified plasmidwas transformed intDH10Bae-host cells, producing specifc trarsposition withBacmid to yield the recombinant shuttle vehicles, rBacmid-ORF5. The baculovirus cpression vector was successfully corerstrutted, which would be useful for proluction of gene vaccination in future.

Key words: Porcine reproductive and respiratory syndrome virus, ORF5 gene, Cloning, Baculovirus expression

CLC Number:

S852.65

ZHAO Na, TIAN Hong, WU Jin-yan, MAN Zi-ping, SHANG Youjun, HE Sheng-hu, LIU Xiang-tao. Cloning of ORFS Gene of PRRS Virus and Construction of Its Baculovirus Expression Vector[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(1): 83-86. doi: 10.7668/hbnxb.2009.01.019.

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