Factors Influencing Microspore Embrogenesis and Construction of Dihaploid Population in Yellow-seeded Brassica napus L.

doi:10.3321/j.issn:1000-7091.2007.01.026

Abstract

Abstract: In China, donor plants for microspore culture usually grow in field and are affected by external environment greatly, leading to low embryogenesis rate. To solve this problem, critical factors, which may have effects on embryogenesis rate were investigated. Meantime, yellow-seeded rapeseed was studied for its response to microspore culture. The results showed that sampling date had tremendous influence to embryogenesis rate, and negative effect of colchicum treatment was detected, with the higher colchicum concentration, the greater influence. Embryogenesis rate of yellow-seeded rapeseed was only about one-eighth of the black-seeded ones. However, the embryogenesis rate among different yellow-seeded plants varied greatly, ranging from 0.04-1.97 embryos per plate. The plant survival rate reached 89.0% by directly transplanting microspore derived plants from culture jars to field. After chromosome doubling, a yellow seed dihaploid (DH) population containing 127 lines was constructed.

Key words: Oilseed rape, Microspore culture, Yellow seed, Dihaploid population

CLC Number:

S634.301

MEI De-sheng, WANG Han-zhong, LI Yun-chang, HU Qiong, LI Ying-de, XU Yu-song. Factors Influencing Microspore Embrogenesis and Construction of Dihaploid Population in Yellow-seeded Brassica napus L.[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(1): 112-115. doi: 10.3321/j.issn:1000-7091.2007.01.026.

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