Test of Preservation, Artificial Insemination and Hatching for Gene Transfer Mirror Carp Sperm

doi:10.7668/hbnxb.2006.S3.014

Abstract

Abstract: Sperm of gene transfer mirror carp is preserved in 0.5% hydrolysis milk protein-Hanks liquid at normal atmospheric(23℃) and refrigerated(4℃) temperature. Observe the activity and sustained time of sperm with microscope regularly. The results showed that the effect of sperm preserved at refrigerated temperature either in activity and sustained time or in preserved time is better than that at normal atmospheric temperature. We had done the artificial insemination and hatching test for the sperm preserved 2 days. The results showed that the sperm preserved (2 days) could have inseminated and hatched completely.

Key words: Mirror carp, Sperm, Preservation, Artificial insemination, Hatching

CLC Number:

LI Zhi-ming, ZHENG Shui-ping, LI Chun-ming, ZHANG He-ping. Test of Preservation, Artificial Insemination and Hatching for Gene Transfer Mirror Carp Sperm[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2006, 21(S3): 72-75. doi: 10.7668/hbnxb.2006.S3.014.

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References

[1] 杜昭宏,彭本初,郑水平,等.鲫鱼精子常温保存试验[J].华北农学报,2003,18(S2):75-77.
[2] 成凤莲,王珊.水库网箱养殖框镜鲤的试验[J].内蒙古农业科技,2005,(7)： 294-295.
[3] 缪丽梅,王俊,刘海涛,等.80:20池塘主养德黄鲤试验[J].内蒙古农业科技,2003,(6): 23.
[4] 郭云锁,周凤武,李卫东,等.岱海大银鱼采卵期间雄鱼活体保存试验[J].内蒙古农业科技,2003,(5)：41.
[5] 贾丽,张凤琴,班顺利,等.池塘主养鲤鱼种高产高效试验[J].内蒙古农业科技,2001,(2): 42~43.
[6] 张凤琴,贾丽,张永胜,等.小体积网箱养殖鲤鱼试验[J].内蒙古农业科技,2001,(2)：44.
[7] 李常建,黄中旺.pH值、温度、渗透压对鲤鱼精子低温保存效果的影响[J].水利渔业,2006,26(3)：26-27.
[8] 沈建忠,江庆.南方鲇精子保存方法的初步研究⑴. 水利渔业,2002,22(5)： 13-15.
[9] 谷巍,李胜忠,蔡林钢,等.Na\K*对高白鲑精子活力影响的初步研究[J].水利渔业,2005,25(2):8-10.
[10] 陈松林,刘宪亭,鲁大椿,等.鲤、鲢、鳙精子低温短期保存研究[J].淡水渔业,1992,(3):3-7.
[11] 严安生,李诗模,王其和.鲤鱼与团头妨精子生理生态特性的研究n .钙、镁对精子活力的影响[J].淡水渔业,1993,23(5):5-7.
[12] 邓岳松,林浩然.鱼类精子活力研究进展[J].生命科学研究,1999,3(4)：271-278.
[13] 严安生,王其和,李诗模.渗透压和钾对鲤、团头鲂精子活力的影响[J].淡水渔业,1993,23(3):19-21.
[14] 李国华,崔宗斌.鱼类精子携带外源基因导人[J].水生生物学报,1996,20(3):242-247.
[15] 刘汉勤,郭文.总DNA介导鱼类基因转移的初步研究[J].水生生物学报,1991,15(3)：286-288.
[16] Stoss J et al. Successful storage of Chilled rainbowtrout spermatozoa for up to 34days.Aquaculture,1983,31:269-274.
[17J Hulata G,et al. Cold storage of carp sperm for short periods. Aquaculture,1979,16:267-269
[18] 连晋,王拴文.虹鳟精子液态低温保存方法[J].淡水渔业,1999,29(12)：16-17.
[19] 杜昭宏,郑水平,彭本初,等.几种转基因鱼、杂交鱼耐盐碱性能测试[J].华北农学报,2003,18(专辑）:68-71.
[20] 郑水平,张建华,张利,等.塔拉干水库鲤鱼的生物学特性研究[J].华北农学报,2003,18 (专辑）：144-147.
[21] 江振莹.稀土元素对鲤鱼亲鱼繁殖性能的影响[J].华北农学报,2003,8 (专辑）:78-80.
[22] 彭本初.内蒙古自治区大中水面资源利用现状及今后渔业发展思路[J].华北农学报,2004,19(专辑）：134-136.
[23] 周凤武,郑水平,郭云锁,等.德国镜鲤与黄河鲤正反交子一代生对比试验[J].内蒙古农业科技,2003,(增刊）:265.
[24] 吴丽梅,任风英,段海青,等.池塘80:20主养德黄鲤高产高效技术U].内蒙古农业科技,2003,（增刊）： 160-161.
[25] 贾振荣.调整渔业结构发展特种水产养殖业之我见[J]. 内蒙古农业科技,2000,(增刊）:36-37.

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