Effect of Exogenous Antioxidants on the Enzymatic Activities in Fruit Peel Tissues under High-temperature Stress

doi:10.3321/j.issn:1000-7091.2005.02.012

Abstract

Abstract: By immersion of fruits in solutions of ascorbic acid (ASA), sodium beneoate (SBN), cysteine (Cys)and vitamin E (_E), the effect of different types of exogenous antioxidants on the activities of 5'-nucleodase, LOX and APX in fruit peel tissues under high-temperature stress was examined with Fuji apples so as to ascertain the mechanism of f ruit resistance to heat stress and explore possible preventive practices.The results indicated that, LOX activity increased but APX and 5'-nucleodase activities decreased as high temperature stresses were accentuated, showing an enhancement of oxidative stress that may cause a decline in membrane stabilization.For example, LOX activity increased by 25.2% but 5'-nucleodase and APX activities decreased by 25.55% and 28.8% on average, respectively, at 48℃ as compared to 25℃, w hich substantially means that high temperature stress could aggravate f ruit oxidative status.However, LOX activity reduced whileAPX and 5'-nucleodase activities rose with fruits pretreated w ith exogenous antioxidants in comparison with the control.Under 48℃stress, LOX activity in fruits treated with 4 kinds of antioxidants reduced by 7.2%-7.9% and there appeared a significant decrease of LOX activity in fruits treated with 2 kinds of combined ASA antioxidants|5'- nucleodase activities were enhanced slightly by treatment with SBN, significantly with _E and Cys and very significantly with two combined ASA antioxidants.Likew ise, APX activities rose significantly or very significantly as compared with the control.In a comparison of different exogenous antioxidants, it could be concluded that treatments with ASA, especially mixed with 6-BA or citric acids, resulted in the best improvement in fruit antioxidative capability.

Key words: High-temperature stress, Apple, Enzymatic activity, Antioxidant

CLC Number:

S661.101

ZHANG Jian-qiang, ZHANG Jian-guang, FU Ai-li, Lü|Ming-xia, ZHAO Yun-zhe. Effect of Exogenous Antioxidants on the Enzymatic Activities in Fruit Peel Tissues under High-temperature Stress[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2005, 20(2): 41-44. doi: 10.3321/j.issn:1000-7091.2005.02.012.

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