Detection of Potato Spindle Tuber Viroid by RT??PCR and Analysis of Its Complete Sequences

doi:10.3321/j.issn:1000-7091.2003.z1.016

Abstract

Abstract: A pair of primers w ere designed and synthesized based on PSTVd gene. The ex cepted size 360bpwas amplified by RTvaluePCR from the infected samples,w hile no amplif ied products w ere obtained from thehealthy t issue samples. The unique amplif ied product was then cloned into the pGEMvalueT vector and sequenced.Comparison of the civ il and foreign nucleotide sequence show ed that the homology are 98%. Acting as the posivaluet ive control of RTvaluePCR,the clone of PCR product helped resolve the problem of storing the viroid source andprevent ing it from spreading. This paved a path for breeding the t ransgenic potato resistant to PST Vd.

Key words: PSTVd, RT??PCR, Detection, Cloning, Sequence analysis

CLC Number:

S432??

WU Zhi-ming, JIA Xiao-mei, XIE Xiao-liang, WEN Chun-xiu, TIAN Wei, ZHANG Qing-liang. Detection of Potato Spindle Tuber Viroid by RT??PCR and Analysis of Its Complete Sequences[J]. ACTA AGRICULTURAE BOREALI-SINICA, doi: 10.3321/j.issn:1000-7091.2003.z1.016.

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