Abstract:
The fungal disease resistant gene,chitinase gene chi5B driven by CaMV 35S promoter,wasst ickily inserted into Hind III site of the vector pCAMBLA1304 that is suitable for Agr obacter ium-mediatedDNA t ransformation. The new constructed plasmid pAHCGG included gus and gfp (reported),npt II (bacterialant ibiotic selection),hph (plant ant ibiot ic selection),and chi5B (fungal disease resistant) gene constructs. Theplasmid could be used by the researches not only on improving techniques of plant genet ic transformation,but a-lso on chi5B gene expression in transgenic apples.
Key words:
Vector const ruct ion,
GFP gene,
Chitinase g ene,
Genet ic t ransformat ion,
Tobacoo
CLC Number:
XIE Xiao-liang, ZHAO He, WEN Chun-xiu. Construction of Resistant Gene Vector pAHCGG and It. s Application on Tobacco Genetic Transformation[J]. ACTA AGRICULTURAE BOREALI-SINICA, doi: 10.3321/j.issn:1000-7091.2003.z1.015.