The Cloning and Analysis of the Conserved Sequence of Ethylene Receptor Gene in Wax Apple

doi:10.3321/j.issn:1000-7091.2003.z1.014

Abstract

Abstract: A ethylene receptor protein gene fragment of about 1.5 kb was obtained by PCR amplifying f romthe genomic DNA of w ax apple (Syzygium samarangense)leaves using a pai r of primers designed according tothe conserved sequence of ethy lene recepto r pro tein gene.This f ragment was linked into the pGEM T-easy vector,then t ransformed the E.coli DH5α.This f ragment was analyzed by rest riction enzyme maping and sequenced.The result revealed that the length of this f ragment was 1400 bp contained tw o introns and three exons.The w hole length of exon was 974 bp,coding 324 amino acids.Sequence compared analysis suggested thatthis f ragment was most similar to the sequence of rice(Oryz a sat iva)ethylene receptor gene(osers),the identitywas 98.68 %.

Key words: Wax apple, Ethylene receptor gene, Sequence analysis

CLC Number:

S68.01

HAN Ji-cheng, FENG Zhi-hong, FANG Xuan-jun. The Cloning and Analysis of the Conserved Sequence of Ethylene Receptor Gene in Wax Apple[J]. ACTA AGRICULTURAE BOREALI-SINICA, doi: 10.3321/j.issn:1000-7091.2003.z1.014.

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