Determining Vegetable Hybrid Seed Purity by Isoenzyme Diagnostic Loci

doi:10.3321/j.issn:1000-7091.2001.01.011

Abstract

Abstract: Ten isoenzymes and seed proteins were analyzed in parental inbreds and corresponding F₁ hybrid seeds of 71 commercial varieties including Chinese cabbage,cauliflower,broccoli,tomato,sweet pepper,cucumber and water melon to determine seed purity.The results showed the species with high polymorphism isoenzyme banding pattern have more diagnostic loci between hybrid and their parental inbreds.Ten isoenzymes appeared different polymorphism.POX,PGM and EST phenotypes at 8 genetic loci were different between F₁ hybrids and their parental inbreds of the four Brassica species,cucumber and sweet pepper.Hybrids of tomato could be identified at two MDH loci and 6-PGD.Genetic stability of the diagnostic loci was analyzed and the obvious variation during seed germination from one to ten days was found.A model was developed to estimate seed purity.

Key words: Hybrid purity, Vegetable crop, Loci, Identify

CLC Number:

S603.6

ZHENG Xiaoying, LI Li, XING Baotian. Determining Vegetable Hybrid Seed Purity by Isoenzyme Diagnostic Loci[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2001, 16(1): 56-62. doi: 10.3321/j.issn:1000-7091.2001.01.011.

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