Isolation and Purification of Heat tolerance Related Isozyme of Phosphoglucomutase (PGM) from Chinsese Cabbage

doi:10.3321/j.issn:1000-7091.1998.04.016

Abstract

Abstract: A heat-tolerance(HT) related phosphoglucomutase isozyme was isolated and purified to homogeneity through a series of steps including ex tract ion,am monium sulphate precipitation ,DEAE-cellulose chromat ography and finally native preparative PAGE.The linear molecular weight of this enzyme was estimated to be about 29 kD by SDS-PAGE.Another PGM isozyme was also purified and was shown to be of the same molecular size.The only difference between the two isozymes appears to be their Rfs on native PAGE.The presence of sulfhydryl reagents was most essential for the stability of PGM during the whole purificat ion processe.

Key words: Chinese cabbage, Phosphoglucomutase isozyme, Protein purification, Microsequencing, Heat tolerance gene

CLC Number:

S634.101

Cui Hongchang, Li Li, Zheng Xiaoying, Samue S. Sun. Isolation and Purification of Heat tolerance Related Isozyme of Phosphoglucomutase (PGM) from Chinsese Cabbage[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1998, 13(4): 86-92. doi: 10.3321/j.issn:1000-7091.1998.04.016.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.3321/j.issn:1000-7091.1998.04.016

http://www.hbnxb.net/EN/Y1998/V13/I4/86

[1]	ZHAO Hongkai, ZHANG Xiaoyi, ZENG Huiyun, DENG Jinghan, CHEN Xiaofeng, SONG Lifen. The Effects of Soil Conditioner on the Soil Physicochemical Properties and Biological Characteristics of Non-Heading Chinese Cabbage [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(S1): 312-319.
[2]	FAN Huifen, SUN Tianjie, SU Weihua, XIAO Fuming, ZHANG Jie, WANG Dongmei. Preparation of GmWRKY50 Protein Antibody and Analysis of Its Protein Level Expression [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(6): 28-34.
[3]	ZHANG Hong, XU Yingli, WANG Chaonan, HUANG Zhiyin, FAN Weiqiang, LI Mei, ZHANG Bin. Development and Validation of Molecular Markers for Resistance to Dry Burning Heart Disease in Chinese Cabbage [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(6): 195-203.
[4]	JIANG Nan, ZHENG Qianqian, LI Qianwen, TU Jian, SONG Xiangjun, SHAO Ying, LIU Hongmei, QI Kezong. Prokaryotic Expression Vector Construction,Expression,Purification and Identification of the APEC Virulence Gene ygeG [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(5): 184-190.
[5]	LEI Qidong, SUN Xudong, XU Huini. *Cloning and Prokaryotic Expression of Arabidopsis AtTCP4* Gene** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(5): 24-28.
[6]	RU Shuhua, HOU Limin, ZHAO Ouya, SUN Shiyou, ZHANG Guoyin, WANG Ling, LIU Lei. Effects of Different Types of Amendment and Addition Rate on Soil Cd Availability and Cd Uptake by Chinese Cabbage [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(S1): 274-281.
[7]	PANG Minhui, ZUO Qiang, ZOU Guoyuan, SONG Daping, GAO Lijuan. Effects of Fermentation Prepared from Vegetables Waste on Growth, Quality of Chinese Cabbage [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(S1): 299-305.
[8]	LIU Xiaodong, LI Haishan, MENG Chuan, WANG Yuhai, WU Fang, MU Jingui, MA Junxian, WANG Mingqiu. *A Rapid Identification Method and Application of a New Brassica napus* CMS in Chinese Cabbage** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(5): 26-31.
[9]	SUN Lijing, LI Qianying, WANG Peinan, SUN Ying. Protein Expression and Purification of OsAHP2 in Rice [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(4): 46-51.
[10]	CHEN Yanan, Lü Xiaohui, DING Qian, ZHENG Han, YANG Ning, SONG Qingqing, WANG Junfeng, LI Jingjuan, GAO Jianwei. Genotypic Difference and Characteristic Analysis of Nitrogen Response in Chinese Cabbage [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(1): 131-140.
[11]	FU Bingkun, LIU Tianli, SUN Mengyao, CAO Zhifu, WANG Qian. Effect of Density on Dynamic Change of Leaf Area Index and Yield of Seedling-edible Chinese Cabbage [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(S1): 119-123.
[12]	SU Jianhui, ZHANG Leqiang, WANG Ruying, WANG Yanhua, ZHANG Weiwei, MA Cong, XUAN Shuxin, SHEN Shuxing. Development and Application of Gene Markers in the Exogenous Chromosome Segment of Chinese Cabbage-Cabbage Translation Lines [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(3): 89-95.
[13]	WANG Chaonan, ZHANG Bin, ZHANG Hong, WEN Juanjuan, WANG Tao. Mapping of Clubroot Resistance Gene and Developing Linkage Markers in Brassica rapa L. ssp. pekinensis [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(3): 203-208.
[14]	WEN Juanjuan, WANG Chaonan, HUA Deping, ZHANG Hong, XU Yingli, ZHANG Bin. *Reaction and Resistant Gene Loci Detection of Different Chinese Cabbage Varieties to Different Plasmodiophora brassicae* Strains** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(2): 103-109.
[15]	CUI Feifei, MENG Chuan, WANG Yanhua, ZHAO Jianjun, CHEN Xueping, SHEN Shuxing, GU Aixia. Reference Genes Selection for Quantitative Real-time PCR in Chinese Cabbage-Cabbage Translocation Lines [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(5): 60-67.

Please choose a citation manager

Content to export