Purity Test of F1 Hybrid Cultivars of Several Vegetables by Seed Water-soluble Protein Isoelectric Focusing Electrophoresis

doi:10.3321/j.issn:1000-7091.1995.04.014

Abstract

Abstract: In this study eight vegetable F₁ hybrid cultivars were clearly distinguished from their parents based on the protein band patterns.A method of testing hybrid seed purity was developed by using seed water-soluble protein isoelectric focusing electrophoresis.The purity test results were attested to be correct by the congentional method of field test which needed several months.The protein electrophoresis is quick, easy, cheap and accurate, as compared with the traditional method and also can be a very useful tool in genetic analysis for vegetable breeders.

Key words: Chineses cabbage, Cabbage Brocoli, Sweet pepper, Hybrid, Parent, Purity, Isoelectric focusing electrophoresis, Seed water-soluble protein

CLC Number:

S603

Huang Weiping, Zheng Xiaoying, Xing Baotian. Purity Test of F₁ Hybrid Cultivars of Several Vegetables by Seed Water-soluble Protein Isoelectric Focusing Electrophoresis[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1995, 10(4): 76-81. doi: 10.3321/j.issn:1000-7091.1995.04.014.

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Purity Test of F₁ Hybrid Cultivars of Several Vegetables by Seed Water-soluble Protein Isoelectric Focusing Electrophoresis

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