Studies on the Nuclear Polyhedrosis Virus Disease of Clanis bilineata tsingtauica Mell

doi:10.3321/j.issn:1000-7091.1993.04.012

Abstract

Abstract: In August 1981, a nuclear polyhedrosis virus (NPV) was isolated from dead larvae of Clanis bilineata tsingtauica Mellin the laboratory. Under electron microscopy the polyhedra were seen as nearly rounded and hexagon in shape, verying from 0.6 to 2.0 micron in diameter. The virus particles liberated from the polyhedra in 0.05 molhL Na₂CO₃and 0.05 mol/L NaCl were rod shape with rounded ends, measured 50×320 nm.Bioassay conducted in the laboratory showed that the medium lethal concentration(LC₅₀) was 1.4×104,8polyhedra/ml, y= 3.24+0.54x. Field tests showed that the mortality of the larvae may reach 70% by spraying with the polyhedral suspension. Mixed application of the CbNPV and PaNPV, or CbNPV and Bacillus thuringiensis, was tested in the field, over 90% mortality was obtained.

Key words: Clanis bilineata tsingtauica, Nuclear polyhedrosis virus (NPV), bacillus thuringiensis, Efficiency of control.

Sun Faren, Wang Huaikuan. Studies on the Nuclear Polyhedrosis Virus Disease of Clanis bilineata tsingtauica Mell[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1993, 8(4): 59-63. doi: 10.3321/j.issn:1000-7091.1993.04.012.

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