Application and the New Development of the Enzyme-linked Immunosorbent Assay to the Detections of Bacillus thuringiensis Insecticidal Crystal Protein

doi:10.7668/hbnxb.2007.S2.051

Abstract

Abstract: Bacillus thuringiensis(Bt) as one of the most widely used microbial pesticide,is a gram-positive bacterium that produces insecticidal proteins as crystalline protoxin inclusions during sporulation,and the Bacillus thuringiensis insecticidal protoxin is the main insecticidal ingredient,and the traditional detecting method of the insecticidal protoxin are bioassay and sodium dodecyl sulfate polyacrylamide gel electrophorasis(SDS-PAGE),but the methods are very complicated,at the same time not very sensitive.So the detecting method need to be improved.In this paper,applications of the Enzyme-linked Immunosorbent Assay(ELISA)to the detections of the Bacillus thuringiensis toxins,types and basic principles of the ELISA detected toxins from the Bacillus thuringiensis were all summarized,and the prospect of the ELISA was also reviewed.

Key words: Bacillus thuringiensis, Insecticidal crystal protein, Enzyme linked immunsorbsent assay

CLC Number:

S476.11

WU Rui-hua, LI Guo-xun, FENG Shu-liang, WANG Rong-yan, WANG Jinyao, CAO Wei-ping, DU Li-xin, CHEN Dan. Application and the New Development of the Enzyme-linked Immunosorbent Assay to the Detections of Bacillus thuringiensis Insecticidal Crystal Protein[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(S2): 216-219. doi: 10.7668/hbnxb.2007.S2.051.

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