Analysis of Key Metabolites and ANS Genes Affecting Seed Testa Color of Peanut

doi:10.7668/hbnxb.20193143

Abstract

Abstract:

In order to explore the major metabolites and the key anthocyanin synthase(ANS)genes which affecting the seed testa color in peanut,here,5 peanut varieties(lines)with different seed testa color were employed to determine the main anthocyanin metabolite,expression pattern of ANS family genes as well as their correlations in seed testa. The results showed that a total of 19 anthocyanin glycosides which belonged to 3 categories were detected in the seed testa of 5 peanut varieties(lines). Meanwhile,a total of 17 anthocyanin glycosides were found in the black seed testa,while only 4 anthocyanin glycosides were detected in white seed testa,indicating that the number of anthocyanin glycosides were gradually increased accompanying the deepening of seed testa color. In addition,the expression analysis of the 5 ANS genes showed that only Ahy_Scaffold1g106620 was highly expressed,while,the other 4 genes were almost not expressed or had a low expression. Furthermore,the expression level of Ahy_Scaffold1g106620 was significantly increased accompanying the deepening of seed testa color,suggesting that Ahy_Scaffold1g106620 was a key gene in regulating of anthocyanin synthesis. Further correlation analysis revealed that Ahy_Scaffold1g106620 was significantly and positively correlated with 12 of the 19 glycosides with correlation coefficients ranged from 0.54 to 0.82. However,Ahy_A02g006729 showed significant correlation with only 3 anthocyanin glycosides,and the other 3 genes had no correlation with anthocyanin content in seed testa. These results demonstrated that Ahy_Scaffold1g106620,a member of ANS gene family,was the vital regulatory gene in accumulating anthocyanin. In summary,all these results preliminarily discovered the main metabolites and key regulatory genes of ANS family that affect the seed testa color in peanut.

Key words: Peanut, Anthocyanidin, ANS genes, Metabolite, Testa color

SU Qiao, JIN Xinxin, LI Yurong, CHENG Zengshu, SONG Yahui, YANG Yongqing, WANG Jin. Analysis of Key Metabolites and ANS Genes Affecting Seed Testa Color of Peanut[J]. Acta Agriculturae Boreali-Sinica, 2022, 37(S1): 19-25. doi: 10.7668/hbnxb.20193143.

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Figures/Tables 5

Fig.1 Analysis of anthocyanins in seed testa of 5 peanut varieties(lines)

Fig.2 Expression analysis of 5 ANS genes in seed testa of 5 peanut varieties(lines)

Fig.3 Correlation analysis of ANS gene expression level and anthocyanin content in peanut seed testa ns.The correlation is not significant;^*.A significant correlation at the 0.05 level;^**.A significant correlation at the 0.01 level;^***.A significant correlation at the 0.001 level.

Fig.4 Candidate gene structure

Tab.1 Isoelectric points and molecular weight statistics of 5 candidate genes

基因 Gene	等电点 Isoelectric point	分子量/ku Molecular weight	亚细胞定位 Subcellular localization	亲/疏水性 Hydrophilic/hydrophobic
Ahy_Scaffold1g106620	5.60	39.929 89	细胞质	亲水
Ahy_A02g006729	6.05	42.190 12	细胞质	亲水
Ahy_B02g061206	5.53	42.637 53	细胞质	亲水
Ahy_B07g087995	5.75	32.739 29	细胞质	亲水
Ahy_B10g102589	6.41	41.000 69	细胞质	亲水

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