Effect of Inorganic Organic Fertilizer Combined with Biochar on Enzyme Activity and Phosphorus Forms in Reclaimed Soil

doi:10.7668/hbnxb.20190240

Abstract

Abstract: To explore the fertilization measures for the poor soil in the coal mining subsidence reclamation area, this paper used the calcareous soil in the coal mining subsidence area of Xiangyuan County, Changzhi City for five years to test the soil enzyme activity and phosphorus form and the variation law was investigated. The results showed that biochar promoted the activity of urease, alkaline phosphatase, invertase, catalase and dehydrogenase in soil during the test period.The highest increase was 647.0%, 223.0%, 68.6%, 93.7%, and 331.0%.Different fertilization treatments, the mixed application of organic fertilizer and chemical fertilizer to promote urease activity in soil is stronger than single application of organic fertilizer or chemical fertilizer, but single application of organic fertilizer has effects on alkaline phosphatase, invertase, catalase and dehydrogenase. The promotion effect is stronger than the single application of chemical fertilizer and the mixed application of organic fertilizer and chemical fertilizer. With the addition of biochar, the content of various phosphorus forms varied significantly among different fertilization treatments. Among them, Ca₂-P and Ca₁₀-P were relatively abundant, which increased by 17.09% and 4.58% respectively; Ca₈-P, Al-P, the content of Fe-P and O-P was gradually depleted, with a maximum reduction of 3.39%, 5.40%, 7.20%, and 3.96%, respectively. The maximum increments of available phosphorus and total phosphorus were 11.0% and 4.34% respectively. The content of inorganic phosphorus was increased to be 53.5%-63.3% higher than CK. The correlation results, the available phosphorus in the reclaimed soil was closely related to the contents of Ca₂-P, Ca₈-P and O-P. In addition, it could be seen that in the reclaimed soil, Ca-P, iron-aluminum bound phosphorus and closed-state phosphorus maintain a certain ratio, and under certain conditions, they could be transformed by Ca₈-P and O-P.

Key words: Reclaimed soil, Biochar, Enzyme activity, Inorganic phosphorus, Available phosphoru

CLC Number:

S154.3

WEI Junjie, HONG Jianping. Effect of Inorganic Organic Fertilizer Combined with Biochar on Enzyme Activity and Phosphorus Forms in Reclaimed Soil[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(6): 170-176. doi: 10.7668/hbnxb.20190240.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.20190240

http://www.hbnxb.net/EN/Y2019/V34/I6/170

References

[1] 李斯佳,王金满,万德鹏,白中科.采煤沉陷地微地形改造及其应用研究进展[J].生态学杂志,2018,37(6):1612-1619.doi:10.13292/j.1000-4890.201806.031.
Li S J, Wang J M, Wan D P, Bai Z K. Micro-landform modification and its application of coal-mining subsidence area:A review[J]. Chinese Journal of Ecology, 2018, 37(6):1612-1819.
[2] 梁利宝,洪坚平,谢英荷,杨彦.不同培肥处理对采煤塌陷地复垦不同年限土壤熟化的影响[J].水土保持学报,2010,24(3):140-144.doi:10.13870/j.cnki.stbcxb.2010.03.028.
Liang L B, Hong J P, Xie Y H, Yang Y.Effect of reclaimed soil on subsided land resulting from Coal-mine by different treatments of application fertilizers with different reclamation years[J].Journal of Soil and Water Conservation,2010,24(3):140-144.
[3] 陈温福,张伟明,孟军.农用生物炭研究进展与前景[J].中国农业科学,2013,46(16):3324-3333.doi:10.3864/j.issn.0518-1752.2013.16.003.
Chen W F, Zhang W M, Meng J. Advances and prospects in research of biocar utilization in agriculture[J]. Scientia Agricultura Sinica, 2013, 46(16):3324-3333.
[4] 郭茹,洪坚平.不同生物炭配施腐植酸对铬污染土壤中油菜品质及铬含量的影响[J].山西农业科学,2018,46(3):397-401. doi:10.3969/j.issn.1002-2481.2018.03.21.
Guo R,Hong J P.Effect of different types of biochar with humic acid on the quality and chromium content of potted cole of chromium cintaminated soil[J]. Journal of Shanxi Agricultural Sciences,2018,46(3):397-401.
[5] 蒲生彦,上官李想,刘世宾,石清清,王晓科,张颖.生物炭及其复合材料在土壤污染修复中的应用研究进展[J].生态环境学报,2019,28(3):629-635.doi:10.16258/j.cnki.1674-5906.2019.03.025.
Pu S Y, Shangguan L X, Liu S B, Shi Q Q, Wang X K, Zhang Y.A Review of the application of biochar and its composites in soil remediation[J]. Ecology and Environmental Sciences, 2019,28(3):629-635.
[6] Tammeorg P, Simojoki A, Mäkelä P, Stoddard F L, Alakukku L, Helenius J. Biochar application to a fertile sandy clay loam in boreal conditions:effects on soil properties and yield formation of wheat, turnip rape and faba bean[J]. Plant and Soil,2014,374(1-2):89-107.doi:10.1007/s11104-013-1851-5.
[7] 李明,李忠佩,刘明,江春玉,吴萌.不同秸秆生物炭对红壤性水稻土养分及微生物群落结构的影响[J].中国农业科学,2015,48(7):1361-1369.doi:10.3864/j.issn.0578-1752.2015.07.11.
Li M, Li Z P, Liu M, Jiang C Y, Wu M.Effects of different straw biochar on nutrient and microbial community structure of a red paddy soil[J].Scientia Agricultura Sinica,2015,48(7):1361-1369.
[8] 顾美英,刘洪亮,李志强,刘晓伟,唐光木,徐万里.新疆连作棉田施用生物炭对土壤养分及微生物群落多样性的影响[J].中国农业科学,2014,47(20):4128-4138. doi:10.3864/j.issn.0578-1752.2014.20.021.
Gu M Y, Liu H L, Li Z Q, Liu X W, Tang G M, Xu W L.Impact of biochar application on soil nutrients and microbial diversities in continuous cultivated cotton fields in Xinjiang[J]. Scientia Agricultura Sinica,2014,47(20):4128-4138.
[9] Mayer Z A, Eltom Y, Stennett D, Schr der E, Apfelbacher A, Hornung A. Characterization of engineered biochar for soil management[J]. Environmental Progress&Sustainable Energy,2014,33(2):490-496.doi:10.1002/ep.11788.
[10] Xu G, Lü Y C, Sun J N, Shao H B, Wei L L. Recent advances in biochar applications in agricultural soils:benefits and environmental implications[J]. CLEAN-Soil Air Water, 2012, 40(10):1093-1098.doi:10.1002/clen.201100738.
[11] Gomez J D, Denef K, Stewart C E, Zheng J, Cotrufo M F. Biochar addition rate influences soil microbial abundance and activity in temperate soils[J]. European Journal of Soil Science,2014,65(1):28-39.doi:10.1111/ejss.12097.
[12] Li D, Hockaday W C, Masiello C A, Alvareza P J J. Earthworm avoidance of biochar can be mitigated by wetting[J]. Soil Biology and Biochemistry,2011,43(8):1732-1737.doi:10.1016/j.soilbio.2011.04.019.
[13] Paz-Ferreiro J, Fu S, Méndez A, Gascó G. Interactive effects of biochar and the earthworm Pontoscolex corethrurus on plant productivity and soil enzyme activities[J]. Journal of Soils and Sediments,2014,14(3):483-494.doi:10.1007/s11368-013-0806-z.
[14] Galvez A, Sinicco T, Cayuela M L, Mingorance M D, Fornasier F, Mondinia C. Short term effects of bioenergy by-products on soil C and N dynamics, nutrient availability and biochemical properties[J]. Agriculture, Ecosystems and Environment,2012,160:3-14. doi:10.1016/j.agee.2011.06.015.
[15] Oleszczuk P, Jośko I, Futa B, Pasieczna-Patkowska S, Pałys E, Kraska P. Effect of pesticides on microorganisms, enzymatic activity and plant in biochar-amended soil[J]. Geoderma,2014, 214-215:10-18.doi:10.1016/j.geoderma.2013.10.010.
[16] 尚杰,耿增超,陈心想,张雯,赵军,王森,王亚萍.生物炭对土壤酶活性和糜子产量的影响[J].干旱地区农业研究,2015,33(2):146-151,158.doi:10.16302/j.cnki.1000-7601.2015.02.024.
Shang J, Zhai Z C, Chen X X, Zhang W, Zhao J, Wang S, Wang Y P.Effects of biochar on soil enzyme activities and millet yield[J].Agricultural Research in the Arid Areas,2015,33(2):146-151,158.
[17] 侯晓娜,李慧,朱刘兵,韩燕来,唐政,李忠芳,谭金芳,张水清.生物炭与秸秆添加对砂姜黑土团聚体组成和有机碳分布的影响[J].中国农业科学,2015,48(4):705-712.doi:10.3864/j.issn.0578-1752.2015.04.08.
Hou X N, Li H, Zhu L B, Han Y L, Tang Z, Li Z F, Tan J F, Zhang S Q. Effects of biochar and straw additions on lime concretion black soil aggregate composition and organic carbon distribution[J].Scientia Agricultura Sinica, 2015, 48(4):705-712.
[18] 赵兰凤,张新明,程根,张丽娟,刘小锋,李华兴.生物炭对菜园土壤微生物功能多样性的影响[J].生态学报,2017,37(14):4754-4762. doi:10.5846/stxb201604220758.
Zhao L F, Zhang X M, Cheng G, Zhang L J, Liu X F, Li H X.Effects of biochar on microbial functional diversity in vegetable garden soil[J].Acta Ecologica Sinica,2017,37(14):4754-4762.
[19] 刘园,Khan M J,靳海洋,白雪莹,谢迎新,赵旭,王慎强,王晨阳.秸秆生物炭对潮土作物产量和土壤性状的影响[J].土壤学报,2015,52(4):849-858. doi:10.11766/trxb201412020611.
Liu Y, Khan M J, Jin H Y, Bai X Y, Xie Y X, Zhao X, Wang S Q, Wang C Y.Effects of successive application of crop-straw biochar on crop yield and soil properties in cambosols[J].Acta Pedologica Sinica,2015,52(4):849-858.
[20] 李洪达,李艳,周薇,吕家珑.稻壳生物炭对矿区重金属复合污染土壤中Cd、Zn形态转化的影响[J].农业环境科学学报,2018,37(9):1856-1865. doi:10.11654/jaes.2018-0167.
Li H D,Li Y,Zhou W,Lü J L.Effects of rice-husk-derived biochar on the morphological transformation of Cd and Zn in mining area soils polluted by heavy metals[J].Journal of Agro-Environment Science,2018,37(9):1856-1865.
[21] 葛春辉,唐光木,徐万里.生物质炭对沙质土壤理化性质及作物产量的影响[J].新疆农业科学,2013,50(6):1108-1114.doi:10.6048/j.issn.1001-4330.2013.06.020.
Ge C H, Tang G M, Xu W L. Influence of application of bio-carbon on characters of soil and crop yield[J].Xinjiang Agricultural Sciences,2013,50(6):1108-1114.
[22] 郭伟,陈红霞,张庆忠,王一丁.华北高产农田施用生物质炭对耕层土壤总氮和碱解氮含量的影响[J].生态环境学报,2011,20(3):425-428. doi:10.16258/j.cnki.1674-5906.2011.03.016.
Guo W, Chen H X, Zhang Q Z, Wang Y D. Effects of biochar application on total nitrogen and alkali-hydrolyzable nitrogen content in the topsoil of the high-yield cropland in north China Plain[J].Ecology and Environmental Sciences,2011,20(3):425-428.
[23] 严正娟.施用粪肥对设施菜田土壤磷素形态与移动性的影响[D].北京:中国农业大学,2015.
Yan Z J. Effect of manure application on the form and mobility of soil phosphorus vegetable in greenhouse[D]. Beijing:China Agricultural University, 2015.
[24] 和文祥,王娟,高亚军,田海霞,曹卫东.不同价态铬的土壤碱性磷酸酶效应模拟研究[J].农业环境科学学报,2010,29(1):104-109.
He W X,Wang J,Gao Y J,Tian H X,Cao W D.Effect of different valences chromium on soil Alk-phosphatase characteritics[J]. Journal of Agro-Environment Science,2010,29(1):104-109.
[25] 李东坡,武志杰,陈利军,杨杰,朱平,任军,彭畅,高红军.长期培肥黑土脲酶活性动态变化及其影响因素[J].应用生态学报,2003,14(12):2208-2212.doi:10.13287/j.1001-9332.2003.0487.
Li D P, Wu Z J, Chen L J, Yang J, Zhu P, Ren J, Peng C, Gao H J. Dynamic of urease activity in long-term fertilization black soil and its affecting factors[J]. Chinese Journal of Applied Ecology, 2003,14(12):2208-2212.
[26] 贾俊香,谢英荷.生物炭对采煤塌陷复垦区土壤养分与酶活性的影响[J].灌溉排水学报,2016,35(11):88-91.doi:10.13522/j.cnki.ggps.2016.11.016.
Jia J X,Xie Y H.Effects of biochar on soil nutrient and enzyme activity from coal mining subsidence reclamation area[J].Journal of Irrigation and Drainage,2016,35(11):88-91.
[27] Novak J, Busscher W, Laird D, Ahmedna M, Watts D, Niandou M. Impact of biochar amendment on fertility of a southeastern coastal plain soil[J]. Soil Science,2009,174(2):105-112.doi:10.1097/SS.0b013e3181981d9a.
[28] 刘继培,刘唯一,周婕,李桐,赵跃,张蒙.施用腐植酸和生物肥对草莓品质、产量及土壤农化性状的影响[J].农业资源与环境学报,2015,32(1):54-59.doi:10.19451/j.cnki.issn1671-9212.2016.02.018.
Liu J P, Liu W Y, Zhou J, Li T, Zhao Y, Zhang M.Effect of applying humic acids and bio-fertilizer on the qualities and yields of strawberry and soil agrochemical characters[J].Journal of Agricultural Resources and Environment,2015,32(1):54-59.
[29] 付丽军,张爱敏,王向东,周国顺.生物有机肥改良设施蔬菜土壤的研究进展[J].中国土壤与肥料,2017(3):1-5. doi:10.11838/sfsc.20170301.
Fu L J, Zhang A M, Wang X D, Zhou G S. Advances on application of bio organic fertilizer for restoring facility vegetable soil[J]. China Soil and Fertilizer, 2017(3):1-5.
[30] 冯瑞兴,施洁君,何胥,金梅娟,沈明星,王海候.水葫芦有机肥对小白菜产量品质及土壤肥力的影响[J].浙江农业科学,2017,58(6):932-936.doi:10.16178/j.issn.0528-9017.20170608.
Feng R X, Shi J J, He X, Jin M J, Shen M X, Wang H H. Effects of water hyacinth organic fertilizer on yield and quality of Chinese cabbage and soil fertility[J].Zhejiang Agricultural Sciences,2017,58(6):932-936.
[31] 张余莽,周海军,张景野,刘淑霞,吴海燕.生物有机肥的研究进展[J].吉林农业科学,2010,35(3):37-40.doi:10.16423/j.cnki.1003-8701.2010.03.003.
Zhang Y M, Zhou H J, Zhang J Y, Liu S X, Wu H Y.Progress of studies of Bio-organic fertilizer[J]. Journal of Jilin Agricultural Sciences,2010,35(3):37-40.
[32] 邢素丽,刘孟朝,徐明岗.有机无机配施对太行山山前平原小麦产量和土壤培肥的影响[J].华北农学报,2010,25(S1):212-216.doi:10.7668/hbnxb.2010.S1.048.
Xing S L, Liu M C, Xu M G.The research of NPK fertilizer combined with soil organic manure enhancing soil fertilization in Taihang piedmont plain[J].Acta Agriculturae Boreali-Sinica,2010,25(S1):212-216.
[33] 孙建,刘苗,李立军,刘景辉.不同施肥处理对土壤理化性质的影响[J].华北农学报,2010,25(4):221-225.
Sun J, Liu M, Li L J, Liu J H.The effect of different fertilization treatments on soil physical and chemical property[J].Acta Agriculturae Boreali-Sinica,2010,25(4):221-225.

Please choose a citation manager

Content to export