Effect of Different Fertilization Treatments on Soil Microbial Function Diversity in Rhizosphere of Sorghum

doi:10.7668/hbnxb.2018.05.027

Abstract

Abstract: Rhizosphere soil microorganisms play an important role in the regulation of soil rhizosphere environment and nutrient transformation. Sorghum has been widely used as forage and strong barren tolerance.Study of rhizosphere microorganisms on the ecological response of different nutrients,which can provide the theoretical basis for nutrient management of different soil nutrients under conditions of sorghum. This paper was based on the long-term field test of sorghum/corn rotation,combined with the pot experiment of artificial greenhouse. BIOLOG micro plate was applied to test the effect of NPK,PK,NK,NP fertilizers and CK (without fertilization) on sorghum rhizosphere soil microbial functional diversity. The results showed that the field test was in agreement with the pot experiment. PK treatment increased the metabolic activity of rhizosphere microorganisms,and improved the utilization of amino acids,carboxylic acids,amines,sugars and polymers,and reduced the utilization of amphiphilic compounds. The Shannon-Wiener index(H),Richness index(S) and Simpson index(Ds) were higher than other treatments. The effects of NK and NP on the functional diversity of rhizosphere microorganisms were not significant,but NK treatment reduced the microbial metabolic activity in the rhizosphere of the late culture. CK treatment significantly decreased the rhizosphere microbial utilization of carbon sources,the Shannon-Wiener index(H),Richness index(S) and Simpson index(Ds) were lower than other treatments,Substrate evenness(E) higher than other treatments. Principal component analysis showed that there was no significant difference in rhizosphere soil microbial carbon source utilization capacity and type between NPK and NP treatments,and the rest treatments showed significant difference in the field test. In pot experiment,the difference of carbon source ability and type of NPK,NK and NP treatment was not significant,and the difference between the other treatments was significant. In summary,fertilization was conducive to improving soil microbial characteristics. The responses of sorghum rhizosphere microorganisms to nitrogen,phosphorus and potassium stress were different. When nitrogen stress was applied,the increase of soil microbial activities in sorghum rhizosphere was probably the cause of nitrogen tolerance in sorghum.

Key words: Sorghum, Different fertilization treatments, Rhizosphere soil, Microbial diversity

CLC Number:

S514

CUI Peipei, WU Ailian, WANG Jinsong, DONG Erwei, NAN Jiangkuan, BAI Wenbin, JIAO Xiaoyan. Effect of Different Fertilization Treatments on Soil Microbial Function Diversity in Rhizosphere of Sorghum[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(5): 195-202. doi: 10.7668/hbnxb.2018.05.027.

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