Effect of Exogenous Hydrogen Sulfide on the Physiological and Biochemical Characteristics of Processing Tomato Seedlings under NaCl Stress

doi:10.7668/hbnxb.2017.01.031

Abstract

Abstract: To investigate the alleviation effect of exogenous hydrogen sulfide on processing tomato seedling growth under salt stress, two processing tomato cultivars (KT-7 and KT-32) with different salt tolerances and treated with different concentrations (20, 50, 100, 150 μmol/L) sodium hydrosulfide processing tomato seedling under 250 mmol/L NaCl stress.The low concentrations of sodium hydrosulfide were used to spray on the leaves of seedling, and to study the seedling biomass, photosynthetic pigment content, relative water content, root activity, free proline accumulation, autioxidant enzyme(SOD, POD, CAT) activities, electrolytic leakage and malondialdehyde (MDA) contents.According to the results, under the salt stress, exogenous H₂S treatment significantly improved the processing tomato seedling biomass, photosynthetic pigment content, relative water content, root activity, proline content and autioxidant enzyme activities. However, electrolytic leakage and MDA contents had a significantly drop. Among them, 50 μmol/L concentration had the best effect. Compared with salt stress alone, 50 μmol/L NaHS increased seedling biomass, chlorophyll a content, chlorophyll b content, carotenoids content, SOD activities by 30.8%-66.7%, 61.8%, 56.3%,57.0%, 9.1%, respectively, however,the electrolytic leakage and MDA contents descend 15 percentage points and 23.7%, in KT-7. 50 mmol/L NaHS increased seedling biomass, chlorophyll a content, chlorophyll b content, carotenoids content, SOD activities by 24.0%-96.4%, 49.8%, 52.0%, 46.7%, 10.2%,respectively, however,the electrolytic leakage and MDA contents descend 12 percentage points and 24.2%, in KT-32.These illustrated that the exogenous H₂S regulated the antioxidant levels in leaves to reduce oxidative damage on processing tomato seedling during salt stress that improved the processing tomato seedling adaptability to salt stress. This study provided the basis for the further study of H₂S function on processing tomato salt stress response mechanism.

Key words: Hydrogen sulfide, Salt stress, Processing tomato, Autioxidant enzyme

CLC Number:

S641.2

ZHENG Zhouyuan, LIN Hairong, CUI Huimei. Effect of Exogenous Hydrogen Sulfide on the Physiological and Biochemical Characteristics of Processing Tomato Seedlings under NaCl Stress[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(1): 208-214. doi: 10.7668/hbnxb.2017.01.031.

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