Abstract: 4-coumarate:coenzyme a ligase(4CL) is a key enzyme for the plant phenylpropanoid derivatives,flavonoids and lignin biosynthetic pathway. In order to study its mechanism on mango fruit,according to the design of degenerate primers of 4CL gene sequence reported,full-length cDNA sequence of 4CL gene of mango fruit was obtained with 3' RACE and 5' RACE method. The full length cDNA sequence of gene was 1 740 bp,the open reading frame was 1 653 bp,encoding 550 amino acids,the molecular weight of 60.47 kDa,isoelectric point 9.51. The 2 318 bp length fragment was amplified from genome and analysis,which contained five introns,respectively,in 1 013-1 139 bp,1 330-1 415 bp,1 564-1 653 bp,1 722-1 802 bp,1 905-2 000 bp. The secondary structure and tertiary structure of 4CL protein were predicted by online software. It was found that the gene encoding for the protein had close relationship with ash,Isatis and other plant through phylogenetic analysis. Expression of 4CL gene in different mango varieties showed:the high expression of the Guifei varieties,and the expression of green Guiqi varieties with low volume. The study showed that the 4CL gene expression was closely related to the mango fruit color.

Key words: Mango, 4CL, Gene cloning

CLC Number: 

• S667.7

• Q78