Gene Cloning and Expression Analysis of VvMSA in Vitis vinifera

doi:10.7668/hbnxb.2016.03.009

Abstract

Abstract: To investigate the function of VvMSA, the full-length cDNA of VvMSA was cloned from Vitis vinifera cultivar Vidal Blanc tissue culture seedlings.Bioinformatic analysis and quantitative Real-time PCR was used to analyze its characteristics.The results showed that VvMSA amplified fragment size of 450 bp encoding 149 amino acids with molecular weight 16.703 kDa,isoelectric point 5.68.VvMSA was an unstable hydrophilic protein and contained a conserved domain ABA/WDS encoding by 65 amino acids.VvMSA and the known homolog LeASR1 in tomato belongs to different sub-families.Real-time PCR analysis showed that the VvMSA was expressed in all tested tissues with the highest expression in flower. VvMSA was induced by salt,drought and cold stress in different levels,and highest expression level was showed after 3 h salt treatment.In addition, VvMSA was also induced by nitric oxide(NO),hydrogen sulfide(H₂S),abscisicacid(ABA) gibberellin(GA₃),auxin(IAA)and ethylene(ET),while salicylic acid(SA)inhibited its expression.The expression pattern of VvMSA by salt treatment was similar with the expression pattern by ABA treatment.In total,these results suggested that the VvMSA was involved in salt resistance by ABA signaling.

Key words: Vitis vinifera, VvMSA, Gene clone, Expression analysis

CLC Number:

Q78
S663.03

ZHANG Suifang, ZHU Dan, MA Qian, HOU Lixia, YIN Pengfei, LIU Xin. Gene Cloning and Expression Analysis of VvMSA in Vitis vinifera[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(3): 58-64. doi: 10.7668/hbnxb.2016.03.009.

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