Study on Abiotic Stress of MdSBP20 Gene from Malus

doi:10.7668/hbnxb.2016.03.008

Abstract

Abstract: To obtain the transcription factor related to stress conditions in apple rootstock,an apple SBP gene with the full-length cDNA was cloned in Qingzhen 1(apomictic crabapple Pingyitiancha( Malus hupehensis Rehd.)× columnar apple strain CO(Malus × domestica Borkh.) and M26 via homology-based cloning method.The gene cloned was named as MdSBP20 .Bioinformatics analysis to the cDNA showed that the full-length cDNA was 1 362 bp,it's open reading frame possessed 1 362 bp,and encoded 454 amino acids,which had obviously SBP -domain and two zinc finger structures(Zn-1,Zn-2) and the bothway nuclear location signal(NLS).Phylogenetic analysis suggested that MdSBP20 gene had the closer relationship with Pyrus bretschneideri (XM_009339726.1)and Malus domestica (XM_008376435.1).Real-time PCR analysis showed that MdSBP20 gene played a role in response to low temperature,drought,and salt tolerance.It was inferred that QZ1 might has higher resistance than M26 no matter in stress of low temperature,drought,and salt.Based on the results in this experiment, MdSBP20 gene had the significantly effects in apple rootstock response to abiotic stress.

Key words: Apple(Malus ), Abiotic stress, MdSBP20 gene, Gene clone, Expression analysis

CLC Number:

Q78
S661.1

SHA Ting, HOU Hongmin, MENG Xiangna, BAI Suhua, ZHOU Aiqin, SHA Guangli, DAI Hongyi, ZHU Jun. Study on Abiotic Stress of MdSBP20 Gene from Malus[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(3): 51-57. doi: 10.7668/hbnxb.2016.03.008.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2016.03.008

http://www.hbnxb.net/EN/Y2016/V31/I3/51

References

[1] Li X,Yin X,Wang H,et al.Genome-wide identification and analysis of the Apple(Malus×domestica Borkh.)TIFY gene family[J].Tree Genetics & Genomes,2015,11(1):1-13.
[2] Jiang Yanjuan,Liang Gang,Yu Diqiu.Activated expression of WRKY57 confers drought tolerance in Arabidopsis[J].Molecular Plant,2012,5(6):1375-1388.
[3] Sun Xiaoli,Li Yong,Cai Hua,et al.The Arabidopsis AtbZIP1 transcription factor is a positive regulator of plant tolerance to salt,osmotic and drought stresses[J].Journal of Plant Research,2012,125(3):429-438.
[4] Shin D,Moon S J,Han S,et al.Expression of StMYB1R-1, a novel potato single MYB-like domain transcription factor,increases drought tolerance[J].Plant Physiology,2011,155(1):421-432.
[5] Klein J,Saedler H,Huijser P.A new family of DNA binding proteins includes putative transcriptional regulators of the Antirrhinum majus floral meristem identity gene SQUAMOSA[J].Molecular & General Genetics,1996,250(1):7-16.
[6] Cardon G,H hmann S,Klein J,et al.Molecular characterisation of the Arabidopsis SBP-box genes[J].Gene,1999,237(1):91-104.
[7] Lännenpää M,Jänönen I,Hölttä-vuori M,et al.A new SBP-box gene BpSPL1 in Silver birch ( Betula pendula)[J].Physiologia Plantarum,2004,120(3):491-500.
[8] Kropat J,Tottey S,Birkenbihl R P,et al.A regulator of nutritional Copper signaling in Chlamydomonas is an SBP domain protein that recognizes the GTAC core of Copper response element[J].Proceedings of the National Academy of Sciences of the United States of America,2005,102(51):18730-18735.
[9] Riese M,Höhmann S,Saedler H,et al.Comparative analysis of the SBP-box gene families in P.patens and seed plants[J].Gene,2007,401(1/2):28-37.
[10] Salinas M,Xing Shuping,H hmann S,et al.Genomic organization,phylogenetic comparison and differential expression of the SBP-box family of transcription factors in tomato[J].Planta,2012,235(6):1171-1184.
[11] Xie Kabin,Wu Congqing,Xiong Lizhong.Genomic organization,differential expression,and interaction of SQUAMOSA promoter-binding-like transcription factors and microRNA156 in rice[J].Plant Physiology,2006,142(1):280-293.
[12] Wang Yi,Hu Zongli,Yang Yuxin,et al.Function annotation of an SBP-box gene in Arabidopsis based on analysis of co-expression networks and promoters[J].International Journal of Molecular Sciences,2009,10(1):116-132.
[13] Miura K,Ikeda M,Matsubara A,et al.OsSPL14 promotes panicle branching and higher grain productivity in rice[J].Nature Genetics,2010,42(6):545-549.
[14] Yamasaki H,Hayashi M,Fukazawa M,et al.SQUAMOSA promoter binding Protein-Like7 is a central regulator for Copper homeostasis in Arabidopsis[J].The Plant Cell,2009,21(1):347-361.
[15] Stief A,Altmann S,Hoffmann K,et al.Arabidopsis miR156 regulates tolerance to recurring environmental stress through SPL transcription factors[J].The Plant Cell,2014,26(4):1792-1807.
[16] Cui Na,Sun Xiaoli,Sun Mingzhe,et al.Overexpression of OsmiR156k leads to reduced tolerance to cold stress in rice ( Oryza Sativa )[J].Molecular Breeding,2015,35(11):1-11.
[17] Zhou Xuefeng,Wang Guandong,Sutoh K,et al.Identification of cold-inducible microRNAs in plants by transcriptome analysis[J].Biochimica et Biophysica acta,2008,1779(11):780-788.
[18] Zhang Huijuan,Zhai Jing,Mo Jibo,et al.Overexpression of rice sphingosine-1-phoshpate lyase gene OsSPL1 in transgenic tobacco reduces salt and oxidative stress tolerance[J].Journal of Integrative Plant Biology,2012,54(9):652-662.
[19] Hou Hongmin,Li Jun,Gao Min,et al.Genomic organization,phylogenetic comparison and differential expression of the SBP-box family genes in grape[J].PLoS One,2013,8(3):e59358.
[20] Wang Min,Wang Qinglian,Zhang Baohong.Response of miRNAs and their targets to salt and drought stresses in cotton ( Gossypium hirsutum L.)[J].Gene,2013,530(1):26-32.
[21] Zhou Xuefeng,Wang Guandong,Zhang Weixiong.UV-B responsive microRNA genes in Arabidopsis thaliana[J].Molecular Systems Biology,2007,3(1):103.
[22] Velasco R,Zharkikh A,Affourtit J,et al.The genome of the domesticated Apple ( Malus domestica Borkh.)[J].Nature Genetics,2010,42(10):833-839.
[23] Livak K J,Schmittgen T D.Analysis of relative gene expression data using Real-time quantitative PCR and the 2^-ΔΔCT method[J].Methods,2001,25(4):402-408.

Please choose a citation manager

Content to export