Molecular Detection and Partial Genome Sequence Analysis of Tomato chlorosis virus in Tianjin

doi:10.7668/hbnxb.2016.02.005

Abstract

Abstract: The purpose of this study was to determine whether the tomato plants in Tianjin was infected by Tomato chlorotic virus (ToCV) and whether variation had occurred in the important genes of ToCV.ToCV isolated in Tianjin was diagnosis at molecular level.The genome sequence of coat protein and heat shock protein had been cloned and analyzed.The results showed that the homology of the CP isolate in Tianjin was 97.3% or more with the representative isolates of different countries and regions.The homology of the Hsp70h isolate in Tianjin was 97.2% or more with the representative isolates of different countries and regions.It is proved that Hsp70h and CP protein are the most conserved proteins.This is the first report of Tomato chlorosis virus occurrence in Tianjin.

Key words: Tomato chlorosis virus, Molecular detection, CP, Hsp70h, Genome sequence analysis

CLC Number:

S436.412

JIN Fengmei, XUE Jun, SONG Jian, YU Haitao, DUAN Hongying. Molecular Detection and Partial Genome Sequence Analysis of Tomato chlorosis virus in Tianjin[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(2): 23-27. doi: 10.7668/hbnxb.2016.02.005.

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