Cloning of Agouti Gene in Yak and Polymorphisms Detection

doi:10.7668/hbnxb.2014.05.011

Abstract

Abstract: In yak,Agouti gene is a potential candidate gene in yak skin's color formation and regulation,investigate in Agouti gene sequence polymophism lay the foundation for revealing yak skin and coat color genetic mechanisms.The presented study using DNA cloning techniques to obtain yak Agouti gene encoding sequences.Using bioinformatics tools to forecast the protein's basic physical,chemical properties,hydrophobicity,signal peptide and secondary structure.The polymorphisms of Agouti gene CDS were identificated by DNA pools and direct sequencing in Yak.The results showed that the obtained Agouti was a 593 bp DNA sequence.Gannan black yak Agouti gene was identical with that of Tianzhu white yak.Also,yak Agouti gene was similar with Bos.The yak Agouti gene was named YAK ASIP and registered number in NCBI was KJ630463.This gene encode a hydrophilic protein which containing 133 amino acids.It contains a clear signal peptide and several phosphorylation sites.The secondary structure of the protein was α helix secondary structure and random coil.The Agouti and related proteins of Bos grunniens,Bos taurus,Ovis aries are close in the phylogenetic tree,which showed highly homology.The gene sequence detection showed that the yakAgouti gene was without any polymorphisms.

Key words: Yak, Agouti gene, Cloning, Bioinformatic analysis, Polymorphisms

CLC Number:

ZHANG Jian-yi, LIANG Chun-nian, WU Xiao-yun, ZHANG Liang-bin, GUO Xian, PEI Jie, YAN Ping. Cloning of Agouti Gene in Yak and Polymorphisms Detection[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(5): 59-65. doi: 10.7668/hbnxb.2014.05.011.

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