Abstract: The study of AP1 gene has theoretical value on shortening juvenility of woody fruit plants.According to the reported AP1 gene sequence of apple primers were designed to amplify the AP1 gene of M.baccata using PCR method.The fragment obtained was 1 010 bp in length.The purified PCR products were connected to the pMD18-T vector.Then the products were transformed into E.coli strain DH5α competent cells to obtain recombinant plasmid through the blue-white screening plasmid length and restriction enzyme digestion.Sequencing and sequence analysis showed that:we obtained a middle fragment of AP1 gene,which has two exons,one introns and the part of two introns.The code area altogether codes 81 amino acids,and the 13-81 amino acids is K-region of the MADS-box gene family-specific.After the amino acid sequence carried out on the homology search in GenBank,the results showed that the amino acid sequence homology with other plants is 67.9%-100%.These results laid the foundation for the molecular mechanism of early-flowering phenomenon.They might have both theoretical value and practical significance in fruit tree breeding.

Key words: Y series Malus baccata, AP1(APETALA1) gene, PCR, Cloning, Sequence analysis

CLC Number: 

• Q78