Genetic Variations of ORF3 Gene of PRRSV Isolates from Changjiang River Basin in China

doi:10.7668/hbnxb.2011.03.040

Abstract

Abstract: The ORF3 genes of 29 PRRSV,isolated from Changjiang river basin in China from 2006,were amplified,cloned and sequenced with RT-PCR for analyzing the genetic variation of PRRSV,and blast with the representative isolates.The results showed that ORF3 of all isolates encode the minor structural proteins GP3,254 amino acids.All 29 PRRSV isolates belonged to the North American genotype,while 84.6%-100% amino acid identity with each other.It shared 85.0%-97.2% sequence identity with VR-2332,the representative North American strains of PRRSV.And it shared 86.6%-100% sequence identity with the highly pathogenic PRRSV.The phylogenetic trees indicated that the 29 PRRSV were further divided into two subgenotypes,28 isolates posses a same branch with the highly pathogenic PRRSV,and HZ24 shares a more closely relationship with RespPRRS vaccine.Meanwhile,there are mutation in the epitopes and the antigenic index of GP3 in all 29 isolates.

Key words: PRRSV, ORF3 gene, Genetic variation, Antigenic analysis

CLC Number:

S855.3

LI Bin, HE Kong-wang, WEN Li-bin, MAO Ai-hua, WANG Xiao-min, ZHANG Xue-han, NI Yan-xiu, ZHOU Jun-ming, XIAO Shao-bo, CHEN Huan-chun. Genetic Variations of ORF3 Gene of PRRSV Isolates from Changjiang River Basin in China[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(3): 204-209. doi: 10.7668/hbnxb.2011.03.040.

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