Genetic Variation Analysis of Seven Geographic Populations of Oedaleus asiaticus B. Bienko from Inner Mongolia based on Mitochondrial 16S rRNA Gene Sequences

doi:10.3969/j.issn.1000-7091.2012.04.010

Abstract

Abstract: In order to understand the population status of Oedaleus asiaticus, one of the most dominant grass- hoppers in inner Mongolia,we investigated the genetic structure and genetic variation of seven populations of O. asi- aticus using mtDNA sequence of 16S rRNA. Partial sequence of 289 bp was obtained through sequencing 16S rRNA gene of 28 individuals from seven geographical populations and 267 bp can be used to analysis through editing and cutting. In the sequences obtained, A ＋ T% was about 69.9% and A ＋ T % was significantly higher than G ＋ C%. Twenty polymorphic sites were detected in the 267 bp nucleotide,accounting for 7.49%,and the most of variation are on the third position of the codon. 18 hapotypes were detected in 28 individuals. The molecular phylogenetic tree was constructed with NJ and UPGMA method using Angaracris barabensis and Myrmeleotettix Palpalis outgroup spe- cies. The phylogenetic trees suggested that different individuals of O. asiaticus from seven geographical populations were located in different clusters, the branches of clusters are parallel and there is no obvious clusters of geograph- ical populations. There is no significant correlation between genetic variation and geographic distance.

Key words: Oedaleus asiaticus, Geographical populations mtDNA 16S rRNA, Genetic variation

CLC Number:

S433.2

GAO Shu-jing, LI Dong-wei, LIU Ai-ping, YAN Zhi-jian, WANG Ning, WEI Yun-shan. Genetic Variation Analysis of Seven Geographic Populations of Oedaleus asiaticus B. Bienko from Inner Mongolia based on Mitochondrial 16S rRNA Gene Sequences[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(4): 54-59. doi: 10.3969/j.issn.1000-7091.2012.04.010.

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