Analysis of Genentic Diversity and Population Structure in Melon (Cucumis melo L. ) Breeding Materials by SSR Markers

doi:10.7668/hbnxb.20102.S1.011

Abstract

Abstract: To explore the genetic background of melon breeding materials,23 polymorphic Simple sequence repeat (SSR) markers were conducted to assess the genetic diversity and population structure among 39 melon breeding materials. 71 allelic variations were detected,the average allele number and Shannon-Weaver index of each primer were 3. 087 and 0. 893. Genetic diversity and population structure analyses revealed that results were basically same,39 melon accessions were clustered into two groups,Cucumis melo ssp. melo and C. melo ssp. conomon. The population structure analyses revealed the genetic composition of the 39 accessions,in which 84. 62% accessions shared a likely single ancestor,and the rest were from mixed ancestors. These results had provided valuable information for breeding and exploit of new melon accessions.

Key words: Melon, Breeding materials, SSR, Genetic diversity, Population genetic structure

CLC Number:

S652.03

WANG Mei-rong, XU Yong, ZHAN Yong-le, GUO Shao-gui, REN Yi, GONG Guo-yi, ZHANG Hai-ying. Analysis of Genentic Diversity and Population Structure in Melon (Cucumis melo L. ) Breeding Materials by SSR Markers[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(S1): 41-46. doi: 10.7668/hbnxb.20102.S1.011.

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References

[1] 季维智,宿兵.遗传多样性研究的原理与方法[M].浙江:浙江科学技术出版社,1999:1-10.
[2] 陈家宽,杨继.植物进化生物学[M].武汉:武汉大学出版社,1994:15-208.
[3] 刘丽华,王立新,赵昌平,等.光温敏二系杂交小麦恢复系遗传多样性和群体结构分析[J].中国生物化学与分子生物学报,2009,25(9):867-875.
[4] 王利锋,李会勇,唐保军,等.20份特异玉米地方品种的SSR遗传多样性分析[J].华北农学报,2009,24(1):125-127.
[5] 张军,赵团结,盖钧镒.中国东北大豆育成品种遗传多样性和群体遗传结构分析[J].作物学报,2008,34(9):1529-1536.
[6] 宗绪晓,关建平,顾竟,等.中国和国际豌豆核心种质群体结构与遗传多样性差异分析[J].植物遗传资源学报,2009,10(3):347-353.
[7] 沈镝,方智远,戚春章,等.西双版纳黄瓜群体遗传多样性的SSR分析[J].园艺学报,2009,36(10):1457-1464.
[8] 段艳凤,刘杰,卞春松,等.中国88个马铃薯审定品种SSR指纹图谱构建与遗传多样性分析[J].作物学报,2009,35(8):1451-1457.
[9] 林德珮.甜瓜植物(Cucumis melo L)分类系统的研究[J].中国西瓜甜瓜,1989(1):11-15.
[10] 乐锦华,施江,赵虎基,等.厚皮甜瓜亲缘关系及纯度的RAPD分析[J].果树科学,2000,17(4):295-299.
[11] 盛云燕,栾非时,陈克农,等.甜瓜SSR标记遗传多样性的研究[J].东北农业大学学报,2006,37(2):165-170.
[12] 程振家,王怀松,张志斌,等.甜瓜遗传多样性的AFLP分析[J].西北植物学报,2007,27(2):244-248.
[13] 徐志红,徐永阳,刘均璞,等.甜瓜种质资源遗传多样性及亲缘关系研究[J].果树学报,2008,25(4):552-558.
[14] 胡建斌,李建吾.甜瓜EST-SSR位点信息及标记开发[J].园艺学报,2009,36(4):513-520.
[15] 赵娜,周晶,杨文才,等.厚皮甜瓜遗传多样性的SSR分析[J].中国瓜菜,2009(5):5-10.
[16] Murry H G and Thomspon W F.Rapid isolation of weight DNA[J].Nucleic Acid Research,1980,8:4321-4322.
[17] Bassam B J,Caetano A G,Gresshoff P M.Fast and sensitive silver staining of DNA in polyacryamid gels[J].Anal Biochem,1991,196(1):80-83.
[18] Yeh F C,Yang R C,Boyle T.Popgene version 1.31 quick user guide[C].Canada:University of Alberta,and Centre for International Forestry Research,1999.
[19] Rohlf F.NTSYS-pc ver.2.2:Numerical Taxonomy System[M].New York:Exeter Publishing,Ltd.,2006.
[20] Pritchard J K,Stephens M,Donnelly P.Inference of population structure using multilocus genotype data[J].Genetics,2000,155(2):945-959.
[21] Warburton M L,Xianchun X,Crossal J,et al.Genetic characterization of CIMMYT inbred maize lines and open pollinated populations using large scale fingerprinting methods[J].Crop Science,2002,42:1832-1840.
[22] Adams M D,Kelley J M,Gocayne J D,et al.Complementary DNA sequencing:expressed sequence tags and human genome project[J].Science,1991,252 (5013):1651-1656.
[23] Wang R,Yu Y,Zhao J,et al.Population structure and linkage disequilibrium of a mini core set of maize inbred lines in China[J].Theor Appl Genet,2008,117(7):1141-1153.
[24] 黄树苹.甜瓜种质资源遗传多样性及亲缘关系研究[D].武汉:华中农业大学,2007.

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