Construction of AFLP Genetic Linkage Map and Detection of QTLs for Bacterial Wilt Resistance in Peanut(Arachis hypogaea L.)

doi:10.7668/hbnxb.2010.06.015

Abstract

Abstract: Eighty—two recombinant lines(RILs)derived from the cross between peanut genotypes Yuanza9 1 02 and Chico were employed as genetic linkage mapping population.324 segregating loci were amplifide by 66 AFLP polymorphic primer pairs.Based on the AFLP data.a linkage map of cultivated peanut was constructed using JoinMap3.0 software, which consisted of 98 AFLP markers involvde in 20 linkage groups and covered a total of 285 cM of the genome with an average interval distance of2.90 cM.Combined with the result of bacterial wilt resistance(BW。)evaluated, 3 QTLs(qBW’I, qBW2, qBWr3)for peanut BW。were detected using QTLNetwork2.0 software.qBIVl andqBIV2 were located in group4 while qBW'3 in group 14.These 3 QTLs formed 2 QTL combinations(qBW'I/qBW'3&qBW'2/qBW73)with additive by additive epistasis effects which contributed to qBIV with 12.81%and 16.56%respectively, and explained 21.62%of the total phonotypic variance for BW’.

Key words: Peanut(Arachis hypogaea L.), AFLP, Genetic linkage map, Bacterial wih resistance, QTL

CLC Number:

S565.2

PENG Wen-fang, JIANG Hui-fang, REN Xiao-ping, LU Jian-wei, ZHAO Xin-yan, HUANG Li. Construction of AFLP Genetic Linkage Map and Detection of QTLs for Bacterial Wilt Resistance in Peanut(Arachis hypogaea L.)[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(6): 81-86. doi: 10.7668/hbnxb.2010.06.015.

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