Isolation and Sequence Analysis of a RetrOtransposon·like Sequence Derived from Agropyron mongolicum Keng

doi:10.7668/hbnxb.2010.06.010

Abstract

Abstract: A Ilew DNA fragment was amplified by PCR from DNA of Agropyron mongolicum Keng and was named MwRRT(GenBank GQ855806.1).Sequencing results showed that MwRRT was composed of 1 005 bp and encoded 260 amino acid residues.The homologies analysis of the amino acid sequence in GenBank showed that：it has 47%identity with putative retrotransposon protein Ty3-gypsy subclass、putative gag·pol precursor and putative polyprotein in Oryza sativa at the 98—255 amino acid；it has 52%identity with putative gag-pol precursor in Sorghum bicolor.

Key words: Agropyron mongolicum Keng, Retrotransposon, Cloning

CLC Number:

S543.9

SHI Feng-min, YUN Jin-feng, ZHAO Yan, ZHANG Rui-xia. Isolation and Sequence Analysis of a RetrOtransposon·like Sequence Derived from Agropyron mongolicum Keng[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(6): 52-56. doi: 10.7668/hbnxb.2010.06.010.

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http://www.hbnxb.net/EN/Y2010/V25/I6/52

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