Molecular Cloning.Characterization and Expression of PODl Gene from Ginkgo blloba L.

doi:10.7668/hbnxb.2010.06.009

Abstract

Abstract: The full-length cDNA sequence of novel peroxidasel gene(designed as GbPODl, NCBI accession humber：FJ599670)were isolated from Ginkgo biloba L.The coding region of the gene is l092 bp long, and its deduced mature protein consists of 329 amino acids with a predicted molecular mass of 35.8 kDa and a pI of 8.10.Phylogenetic tree analysis revealed that GbPODl shared the same ancestor with other PODs, could be classified as type III peroxidase.The resuh of southern analysis showed that GbPODl gene was encoded by a multi-gene family in Ginkgo biloba.The expression analysis by RT-PCR showed that GbPODl expressed in a tissue—specific manner in G.biloba.GbPODl was also found to be up-regulated by MeJA, CuS04, CdCl2, MV, and mechanical wounding.These resuhs indicate that the GbPODl has the potential to play a role in response to heavy metal and wounding against foreign attacks, was amultifunction gene.

Key words: Ginkgo biloba L., Peroxidase J, Expression analysis

CLC Number:

S792.95

CHENG Hua, LI Lin-ling, WANG Yan, CHENG Shui-yuan. Molecular Cloning.Characterization and Expression of PODl Gene from Ginkgo blloba L.[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(6): 44-51. doi: 10.7668/hbnxb.2010.06.009.

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