Cloning and Bioinformatics Analysis for a AmERF Gene from Ammopiptanthus mongolicus

doi:10.7668/hbnxb.2010.05.016

Abstract

Abstract: To study the structure and function of ERF,one pairs specific primers were designed,then a new gene AmERF was isolate and cloned from Ammopiptanthus mongolicus genome by PCR technology based on analysis of cDNA Libraries and EST of AmERF. The results indicated that the full-length of AmERF DNA was 899 bp,without intron,had an open reading frame of 633 bp,encoding a predicated polypeptide of 211 amino acids with a molecular weight of 23. 2 kDa and a calculated pI of 8. 2. The study of AmERF gene in the process of biotic stress could provide effective candidate genes for resistance breeding of crop.

Key words: Ammopiptanthus mongolicus, ERF gene, Cloning

CLC Number:

S792.99

LI Xiaodong, BAI Chen, GUO Jiufeng, YU ZhuoSUN Guoqin, ZHANG Huizhong. Cloning and Bioinformatics Analysis for a AmERF Gene from Ammopiptanthus mongolicus[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(5): 76-79. doi: 10.7668/hbnxb.2010.05.016.

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