Isolation,Identification and Biological Characterization of Swine Influenza Viruses in Henan

doi:10.7668/hbnxb.2010.04.023

Abstract

Abstract: Samples were collected by cotton swabs and lung tissues from pigs with respiratory system problems on 5 farms in Henan province. Samples were inoculated into 10-day-old SPF-embryonated eggs via the allantoic route，after being processed. Three isolates were obtained and identified as influenza A virus subtype H3N2 by HA and HI. Some biological properties of them were studied. 50% egg infective doses (EID₅₀) were 10 ^{－9. 56} /0. 2 mL，10 ^{－8. 56} /0. 2 mL and 10 ^{－8. 44} /0. 2 mL. 50% lethal doses (LD₅0) to small white mice of the strain were 10 ^{－1. 56} /0. 1 mL，10^{－1. 45} /0. 1 mL and 10 ^{－1. 50} /0. 1 mL ，respectively. Hemagglutinins of the strains were unstable to heat. Red blood cells of horse， cattle，pig，donkey，pig，sheep，cock，rabbit，guinea pig，small white mouse，big white mouse and human O-type could be agglutinated by the isolate. It will lay a foundation for understanding influenza viruses circulating in swine herds in Henan province.

Key words: Swine influenza virus, Isolation, Identification, Biological properties

CLC Number:

S32.+1

ZHAO Pu, ZHENG Yu-shu, JIA Bei-bei, LIU Li-yan, LIU Xing-you, LI Hai-yan. Isolation,Identification and Biological Characterization of Swine Influenza Viruses in Henan[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(4): 107-110. doi: 10.7668/hbnxb.2010.04.023.

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