Correlation of H-FABP Gene Expression with IMF and Fatty Acid Content in Laiwu Pigs and Duroc

doi:10.7668/hbnxb.2010.04.014

Abstract

Abstract: This experiment was conducted to study the differential expression of H-FABP gene between Laiwu pigs and Duroc，and analysis correlation of H-FABP gene expression with IMF(intramuscular fat) and fatty acid content of longissimus muscle. Ten Laiwu pigs and seven Duroc weighting approximately 100 kg were chosen for the experiment. Expression of H-FABP at mRNA in LM of Laiwu pigs and Duroc were measured by real time fluorescence RT-PCR，I MF and fatty acid content were measured. The result indicated that，Compared with Duroc，H-FABP gene expression of longissimus muscle in Laiwu pigs was increased by 36. 16% . Correlation between H-FABP gene expression and IMF content with SFA(saturated fatty acid) ，SFA + MUFA (monounsaturated fatty acids) in Laiwu pigs Duroc was prominent. Correlation between H-FABP gene expression and IMF content with PUSA(polyunsaturated fatty acid) was distinctly negative，with sum of fatty acid in Laiwu pigs was not remarkable，t hat's of Duroc was contrary. Correlation between H-FABP gene expression with IMF content and fatty acid composing was marked，so H-FABP is candidate gene of IMF in Laiwu pigs＇ breeding.

Key words: Laiwu pigs, Real-time fluorescence RT-PCR, H-FABP gene expression, IMF, Fatty acid

CLC Number:

HU Hong-mei, WANG Ji-ying, GUO Jian-feng, ZHANG Yin, SHEN Yan-feng, WU Ying. Correlation of H-FABP Gene Expression with IMF and Fatty Acid Content in Laiwu Pigs and Duroc[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(4): 64-68. doi: 10.7668/hbnxb.2010.04.014.

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