The Effect of Adding Protozoa on Metabolism and Activity of Rumen Bacteria

doi:10.7668/hbnxb.2018.S1.044

Abstract

Abstract: Rumen is like a fermentation tank where ruminant animals digest feeds. The complex microbes hosted in rumen mainly comprise bacteria,protozoa,and fungi. Interaction of rumen microbes provided a reliable guarantee for feed digestion and absorption. The objective of this trial was to study the effect of rumen protozoa on bacteria activity and further explore whether rumen protozoa stimulate or maintain the activity of rumen bacteria.Two castrated Simmental bulls fitted with permanent rumen fistulas were used as the donors of rumen fluid and Chinese wildrye were used as fermented inocula. The trial was grouped into three treatments containing bacteria group,protozoa group,and bacteria+protozoa group,respectively. The fermentation pattern of feeds was determined using in vitro incubation technique for 24 h. The pH value was measured immediately after incubation. Gas component productions such as methane,carbon dioxide and hydrogen,and individual volatile fatty acid production was determined by gas chromatograph. Results indicated that the total gas production of bacteria+protozoa group was significantly greater than bacterial group (P<0.05), whereas the gas production of protozoan group was zero. The total VFA, acetate, propionate, and butyrate production of bacteria+protozoa group were extremely significantly greater than those in the bacterial group or protozoan group (P<0.01), which indicated that the fermentation efficiency of bacteria+protozoa group was greater than monoculture of protozoa or bacteria,thus demonstrated that protozoa promoted rumen bacteria activity during the degradation and fermentation of plant cell wall. It also revealed that degradation of plant cell wall in the rumen was conducted under the combined function of bacteria and protozoa,which showed that there was a synergistic effect between rumen bacteria and protozoa.

Key words: Rumen bacteria, Protozoa, in vitro incubation technique, Volatile fatty acid

CLC Number:

S432.4

DONG Ruilan, YU Guanghui. The Effect of Adding Protozoa on Metabolism and Activity of Rumen Bacteria[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(S1): 278-284. doi: 10.7668/hbnxb.2018.S1.044.

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