The Studies on Constructing Expression Vector of Ammopiptanthus mongolicus Dehydrin Gene and Genetic Transformation to Sugar Beet

doi:10.7668/hbnxb.2010.01.013

Abstract

Abstract: In order to study function of Ammopiptanthus mongolicus dehydrin gene,First,the primers were designed according to the sequence of Ammopiptanthus mongolicus dehydrin cDNA library,the dehydrin gene was amplified with PCR,then carried out site-directed mutagenesis for its a base internal,and cloned into pCAMBIA3301 vector.The dehydrin gene was introduced into sugar beet by Agrobacterium mediated tumerfaciens,and resistance to Km of Sugar beet were obtained,which lays a good foundation for the further research.

Key words: Dehydrin, Cloning, Site-directed mutagenesis

CLC Number:

Q785

LI Xiao-dong1,2,BAI Chen1,ZHANG YU-jin2,YU Zhuo1,SUN Guo-qin2,ZHANG Hui-zhong2,GUO Jiu-feng2,KANG Lian-he2. The Studies on Constructing Expression Vector of Ammopiptanthus mongolicus Dehydrin Gene and Genetic Transformation to Sugar Beet[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(1): 68-74. doi: 10.7668/hbnxb.2010.01.013.

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