Prokaryotic Expression of Ecoli Heat -labile Enterotoxin LTa and LTb and Its Immunogenenity in Mice

doi:10.7668/hbnxb.2009.S1.062

Abstract

Abstract: Ecoli heat -labile enterotoxin is a best immune adjuvant for its immunic enhancement.In order to studythe effect of its immunic enhancement,pGST -LTa(R210G)and pGST -LTb was constructed and expressed,the resultof western -blotting showed the recombinant protein of GST -LTa(R210G)and GST -LTb had good biological activitiesanalogy to LT positive sera from pigs.We immunized mice with Ovalbumin,using GST -LTa(R210G)and GST -LTb asimmunoadjuvant,and aluminum hydroxide adjuvant as controls.GST -LTa(R210G)showed the capability to remarkablyincrease the level of antibody against ovalbumin when comparing with those mice without any immunoadjuvant,and whichwas even higher than that of aluminum hydroxide adjuvant,LTb and LTa (R210G)plus LT adjuvant.LTb and LTa(R210G)plus LT adjuvant had the same level of antibody against Ovalbumin.Therefore,LTb and LTa (R210G)can becomea new type of immunoadjuvant in future.

Key words: Ecoliheat-labileenterotoxin, LTaandLTb, Prokaryoticexpression, Immunogenecity

CLC Number:

S858.28

YUANXiu-fang, WANGYi-cheng, XULi-hua, LIUDeng, NIUDeng-yuan, WANGChao-wen. Prokaryotic Expression of Ecoli Heat -labile Enterotoxin LTa and LTb and Its Immunogenenity in Mice[J]. ACTA AGRICULTURAE BOREALI-SINICA, doi: 10.7668/hbnxb.2009.S1.062.

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