ACTA AGRICULTURAE BOREALI-SINICA

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Identification of the Root -knot Nematode from Vegetables in Greenhouses in Shandong

FANYing-lun1, ZHANGWei-guo1, LUShan-hua1, GAOXin-yan2, LIULi-ke1   

  1. 1. College of Agronomy, Liaocheng University, Liaocheng 252059, China;
    2. Agricultural Bureau of Jimo, Jimo 266200, China
  • Received:2009-01-17 Published:2009-12-31

Abstract: Root -knot nematodes(Meloidogyne spp.)are important endoparasitic pests of numerous crop species.Most vegetables are highly susceptible host of several species of root -knot nematodes and incurs yield losses from root -knot infection.The yield of vegetables in greenhouse is increaseing,but nematodes in greenhouse and other controlled environmentproduction systems propagate rapidly.To ascertain the species of the root -knot nematodes ,fourteen samples of root-knot nematodewere collected in the greenhouse planted with various vegetables from seven city of Shandong Province.Thesequences of ITS region of nematode were amplified by PCR.The LTS sequence of nematode collected was compared withthe data of NCBI by BLAST.The results showed that all samples of root -knot nematodes belong to Meloidogyine incognita.

Key words: Vegetable, Meloidogyineincognita, ITSsequence

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Cite this article

FANYing-lun, ZHANGWei-guo, LUShan-hua, GAOXin-yan, LIULi-ke. Identification of the Root -knot Nematode from Vegetables in Greenhouses in Shandong[J]. ACTA AGRICULTURAE BOREALI-SINICA, doi: 10.7668/hbnxb.2009.S1.061.

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