Construction of Subclone Library for Wheat Megabase BAC DNA

doi:10.7668/hbnxb.2009.04.005

Abstract

Abstract: BAC subclone library is an important platform for sequencing the whole genome or some specific regions. In order to accelerate the process of sequencing the wheat genome, a BAC clone (BAC790010) fromLangdon librarywas selected as material, and then was broken by DNA shearing instrument to produce many DNA fragments with different sizes, fromwhich the 3- 5 kb DNA fragments were retrieved and cloned to TOPO vector and then the subclone librarywas constructed that was suitable for shotgun sequencing. This BAC subclone librarywould provide a platformfor gene cloning and genome research of wheat.

Key words: Wheat, BAC clone, Subclone library

CLC Number:

Q785

GAO Shuang-cheng, WANG Shi-hua, SHI Jiang, KONG Xiang-sheng, SHI Guo-an. Construction of Subclone Library for Wheat Megabase BAC DNA[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(4): 23-25. doi: 10.7668/hbnxb.2009.04.005.

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http://www.hbnxb.net/EN/Y2009/V24/I4/23

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