Cloning and Sequence Analysis of a CsEXP5 Gene Fragment from Cucumber Fruits

doi:10.7668/hbnxb.2008.01.003

Abstract

Abstract: A new cDNA fragment of 480 bp was amplified by RT -PCR from total RNA of cucumber young fruits afterpollination. Sequence analysis showed that the cDNA fragment was the same gene to CsEXP5(AF319471)from cucumberroots, and a 526 bp cDNA fragment was obtained by splicing.The predicted CsEXP5 protein contains a series of conservedTry and HFD, KNFRV conserved domains. CsEXP5 was ? -expansin and shares 90% identity with CsEXP10. CsEXP5might be correlated with the expansive growth of cucumber fruit.

Key words: Cucumber fruits, CsEXP5 gene, Sequence analysis

CLC Number:

S642.2

SUN Yong-dong, ZHANG Xing-guo, LI Xin-zheng, LI Zhen-xia, CHEN Bi-hua. Cloning and Sequence Analysis of a CsEXP5 Gene Fragment from Cucumber Fruits[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(1): 12-14. doi: 10.7668/hbnxb.2008.01.003.

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