Use of Random Amplified Polymorphic DNA(RAPD)Analysis to Detect Jujube Witches’ Broom Phytoplasmas

doi:10.7668/hbnxb.2007.05.041

Abstract

Abstract: Jujube witches' broom is a devastating disease of Ziziphus jujube that occurs in various jujube regions of China. Nucleic acid extracted from midribs of samples collected from three jujube varieties (Suanzao, Lajiaozao, Langzao) from symptomatic and asymptomatic shoots were tested by random amplified polymorphic DNA analyses. Using 13 different 10 and 11 bp random primers the amplification of jujube DNA was achieved from all the samples; AM-14 primer provided amplification of specific DNA fragments of about 400 bp, only from samples collected from symptomatic plants. No genetic variations in these varieties were identified using the other 11 arbitrary primers; only with primer AL-07 it was possible to differentiate Lang-zao from the other two varieties tested. All the experiments were repeated 2 times and the results were consistent. Compared with PCR analyses with phytoplasma-specific primers, RAPD techniques resulted to be an alternative rapid and sensitively method for detecting jujube phytoplasmas presence in different jujube varieties.

Key words: Jujube witches’broom, Phytoplasmas, Random Amplified Polymorphic DNA, Detection

CLC Number:

S436.6

FAN Xin-ping, TIAN Jian-bao, A.Bertaccini, WANG Juan, YANG Xiao-yu. Use of Random Amplified Polymorphic DNA(RAPD)Analysis to Detect Jujube Witches’ Broom Phytoplasmas[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(5): 172-175. doi: 10.7668/hbnxb.2007.05.041.

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