Establishment and Optimization of AFLP System for Gramineous Crop Genome

doi:10.7668/hbnxb.2007.04.025

Abstract

Abstract: After studying the four factors of selective amplification, Mg²⁺ concenctration, dNTP concenctration, primer concenctration and dilution times of pre-amplified product, the optimized system of AFLP for wheat was built: taking 2 μL of 20 times dilution of preamplification products as amplification template, adding 0.4 μmol/L of selective primer combinations, 1.5 mmol/L of MgCl₂, 0.2 mmol/L of dNTPs and 1U of Ex Taq polymerase. The AFLP system was confirmed by analyzing the genomic DNA of maize and rice. The results laid a foundation for broadly using the AFLP technology in genetic analysis of gramineous crops.

Key words: AFLP, Selective amplification, Gramineous crop, Wheat, Rice, Maize

CLC Number:

JIAO Zhen, SUN Ying, LI Na, QIN Guang-yong. Establishment and Optimization of AFLP System for Gramineous Crop Genome[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(4): 108-111. doi: 10.7668/hbnxb.2007.04.025.

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